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1. Introduction
2. Overview of lecture: introduction
3. Challenges of immune recognition
4. Antigen recognition has unique features
5. Overview of lecture: key binding concepts
6. Affinity
7. Kinetics
8. Factors affecting affinity
9. Affinity & kinetic constants for key immune interactions
10. Avidity
11. Multivalent high-avidity molecules
12. Overview of lecture: measuring binding properties
13. Methods for measuring binding properties
14. Principles of surface plasmon resonance
15. Measuring the affinity constant (1)
16. Measuring the affinity constant (2)
17. Scatchard plot
18. Measuring affinity by SPR: an example
19. Measuring the dissociation rate constant
20. Measuring the association rate constant
21. Example of measuring binding kinetics
22. Measuring avidity
23. Overview of lecture: cell surface molecules
24. Cell surface molecule interactions are distinct
25. Consequence of constraint to membranes
26. Consequence of mechanical forces
27. Consequence of cooperativity
28. Cooperative binding example
29. CD2/CD48 complex size affects TCR binding
30. Conclusions
31. Acknowledgments

Topics Covered

• Challenges of immune recognition
• Key binding concepts
• Affinity
• Kinetics
• Avidity
• Measuring binding properties
• Cell surface molecules

Links

Series:

• The Immune System - key concepts and questions

Categories:

• Cell Biology
• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on November 30, 2020

Financial Disclosures

• Prof. Anton van der Merwe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.