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Why in vitro permeation test – and not in vivo?

Published on August 31, 2023   24 min

Other Talks in the Series: Periodic Reports: Advances in Clinical Interventions and Research Platforms

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0:00
This is Howard Maibach in San Francisco trying to bring you up to date about an ancient technique from the previous century in vitro per cutaneous penetration test or in vitro permeation test and why it's important and what is the advantages are.
0:23
We can thank the United States Food and Drug Administration for rescuing it and bringing it up to date. Obviously in the load data especially in the animal that we're most interested in namely usually the Homo sapiens has many advantages. But is in many laboratories, awkward because it requires human volunteers and it requires a great deal of regulatory work. The IVPT has been around since before and during the second world war. It has many many purposes which we will go into and can be adapted into many laboratories.
1:11
Its history starts in 1939 when a professor of internal medicine used it to study skin physiology, namely insensible of water loss. The water that comes through your skin at rest in vivo in man it's now called not insensible water loss today it's called TEWL, trans-epidermal water loss. In 1939, also brought the chaos of the second world war. Many countries were very concerned that the chemical warfare agents that created such chaos in Belgium in World War I would be used again in World War II. That led the English government to start the laboratory of Dr. Traeger in 1939. Dr. Traeger developed a stationary in vitro system which was used until the present time and is still being refined. His textbook, if you can get a copy of it, is essentially the equivalent of the Bible, the Old and the New Testament. He foresaw many of the refinements that followed is work. Originally, it was used for chemical warfare agents but now it's used for all types of assays to determine relevance to man.
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Why in vitro permeation test – and not in vivo?

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