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Navigable Slide Index

1. Introduction
2. Multiple myeloma (MM) - Overview
3. Multiple myeloma (MM) - Symptoms
4. Multiple myeloma (MM) - Diagnosis
5. Treatment of MM
6. Treatment of MM - Drug resistance (DR)
7. Bone marrow (BM) microenvironment
8. Exosomes - Overview
9. Exosomes in cancer and as biomarkers
10. Exosomes - Isolation procedures
11. Exosomes - Ultracentrifugation
12. Exosomes - Gradient centrifugation
13. Exosomes - Size exclusion chromatography
14. Exosomes - Immunomagnetic isolation
15. Exosomes - Exoquick kit
16. Exosomes - Confirmation
17. BM - Angiogenesis, Osteolysis, Drug resistance
18. Exosomes in MM - Angiogenesis
19. Investigating MM biology - Models
20. Characterisation of MM exosomes
21. Exosome cargo
22. Role of hypoxia
23. Hypoxia resistant MM cells stimulate angiogenesis
24. miR135b cargo in MM exosome vesicles (EVs)
25. miR340/365 in BM EVs: angiogenesis suppression
26. Exosomes in MM - Osteolysis
27. MM exosomes are taken up by osteoclasts
28. MM exosomes stimulate osteoclasts
29. MM exosomes support osteoclast survival
30. MM exosomes stimulate osteoclasts via IL32
31. MM exosomes inhibit osteoblasts
32. Exosomes in MM - questions about drug resistance
33. BMSC exosomes are picked up by MM cells
34. Migration & proliferation of MM cells
35. BMSC exosomes induce survival of MM cells
36. BMSC induce resistance to bortezomib
37. Drug resistance - Exosome cargo
38. Healthy BMSC exosomes inhibit MM growth
39. Role for IL6 and fibronectin in MM growth
40. Role for miR15a in BMSC EVs in MM suppression
41. miR146a transfer from MM cells to BMSC
42. miR146a mimics increase MM viability & migration
43. MM exosomes induce BMSC to CAF transition
44. Role for miR21/146a
45. Exosomes in MM - Conclusion (recap)
46. Drug exposure increases exosome secretion
47. Chemoexosomes & more heparanase on surface
48. Heparanase in MM cells & cell cycle regulation
49. Exosomes in MM - biomarkers
50. Take home message
51. Acknowledgments

Topics Covered

• Introduction to the biology of Multiple Myeloma
• The development of drug resistance in Myeloma
• The role of the bone marrow environment in drug resistance
• The concept of exosomes
• Points of attention when dealing with exosomes
• The role of exosomes within the bone marrow environment

Links

Series:

• Periodic Reports: Advances in Clinical Interventions and Research Platforms

Categories:

• Cancer
• Cell Biology

Therapeutic Areas:

• Haematology
• Oncology

Talk Citation

Menu, E. (2018, December 30). The role of exosomes in multiple myeloma development & drug resistance [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved February 19, 2019, from https://hstalks.com/bs/3860/.

Publication History

• Published on December 30, 2018