Interviewer: Professor Liu, thank you for taking the time today to discuss this diagnostic test for COVID-19 being developed by yourself and your team.
Could you give an overview of the technology used in this test?
Dr. Liu: Okay. So, recently our lab has developed an All-In-One Dual CRISPR-Cas12a technology
(we also call it "AIOD-CRISPR" diagnostic system).
This diagnostic system has been used for rapid,
sensitive, and specific detection of novel coronavirus SARS-CoV-2,
the causative agent of the COVID-19 disease.
In our AIOD-CRISPR diagnostic system,
we have proposed a dual CRISPR detection strategy by introducing two guide RNAs.
In addition, we combine the recombinase polymerase amplification
(RPA isothermal amplification)
with CRISPR based detection and develop an All-In-One CRISPR-based diagnostic system.
In our AIOD-CRISPR diagnostic system, all reagents for reverse transcription,