DNA damage, mutations and aging 2

Published on July 31, 2017   28 min

Other Talks in the Series: Aging

So the next question for us was, how can we, first of all, escape the limits of that reporter and really look at the genome overall? There are so many interesting features in a genome and we really want to see where the mutations are because maybe the reporter is not such a great measure for the genome overall, and there are all sorts of differences maybe between genes and regulatory sequences. And second, how can we expand our studies, or in general how can we expand studies of somatic mutations to humans, from experimental animals to humans? The first thing we tried is basically try to hitchhike on data that were available, that were collected by others. And it's a student in the lab, another student Brandon Milholland, who started to do this. Brandon began to use what we called
the Cancer Genome Atlas after the emergence of next generation sequencing, so the capacity to really sequence the genome or a substantial part of the genome at low cost and very quickly and efficiently. That was used, that technology, for sequencing tumors because a tumor obviously originates from one cell, it's essentially a clone. So you can take that tumor and basically sequence the genome and they have all the mutations that vary in that original cell when it was still normal, but also that started to accumulate in the tumor itself once it was already a tumor. That's all put together in the Cancer Genome Atlas so others have collected the data, we didn't collect it. But Brandon could access it, because it is free, you can look at it.