Humoral innate immunity and the acute phase response 2

Published on December 18, 2012 Updated on August 31, 2016   42 min

Other Talks in the Series: Cytokines

0:03
Another class of components of the humoral innate immunity is represented by pentraxins. And here, I will focus my attention on pentraxin 3, PTX3. PTX3 was originally cloned in a gene fishing expedition as in IL-1, or LPS, or TNF inducible gene.
0:26
This slide is a schematic representation of the extraction of PTX3, its genomic organization, transcript, and protein. And the molecule was cloned by us. And we cloned a mouse and the human cDNA in genomic and characterized the promoter. PTX3 is a soluble protein consisting of a pentraxin domain encoded by the third exon, coupled with an unrelated and terminal portion. The model here suggests the pentraxin domain holds with a jelly-roll type of the topology. As other pentraxins, PTX3 forms multimers, but in contrast to the classic short pentraxins, which form a pentamer, PTX3 is organized as an octamer. And intra and inter-chain disulfide bridges play a key role in the multimeric organization of PTX3.
1:21
The pentraxin domain of PTX3 is related to the classic short pentraxins, which include the C-reactive protein and the serum amyloid P component. C-reactive protein is a classic acute phase protein in humans. We were lucky because we cloned the first member of the long pentraxin family in which a pentraxin domain is coupled with unrelated and terminal portions. This slide emphasizes, in particular, that we recently cloned another member of the family in silico, in physical, that we called PTX4. This slide also shows an evolution of the analysis of the pentraxin superfamily, indicating that pentraxins are ancient in evolution. On the right side, one can see the toll fish and drosophila. And that the mother of all pentraxins was most likely a long pentraxin.
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Humoral innate immunity and the acute phase response 2

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