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Printable Handouts
Navigable Slide Index
- Introduction
- Agrobacterium tumefaciens
- Agrobacterium infection and the Ti plasmid
- Infection and colonization
- Transfer, integration and expression of T-DNA
- Function of some transfered genes
- Evolution of T-DNA transfer from conjugation
- Using Agrobacterium to create "transgenic" plants
- Expression of toxin from Bacillus in plants
- Detection of plant-released signals by VirA-VirG
- Representative Vir-inducing phenolic compounds
- VirA structure and function
- VirH2 activity
- Quorum sensing
- LuxR-LuxI system of Vibrio fisheri
- TraR-TraI system of Agrobacterium
- Activation of repABC system
- Regulation of Ti plasmid copy number
- Synthesis of AHL by TraI
- Agrobacterium AttM protein
- AttM is part of the attKLM operon
- TraR-autoinducer complex
- TraR as a class I or class II activator
- Purification and solubility of TraR
- OOHL stabilzes TraR
- Formation of TraR-OOHL-DNA complex
- Crystal structure of TraR-AHL-DNA complex
- AHL is engulfed by TraR to it's hydrophobic core
- TraR activity is antagonized by TraM
- Binding of TraR to DNA with dyad symmetry
- Essential bases in TraR binding site
- Affinity of TraR for wild type tra box and variations
- TraR residues required for transcription activation
- TraR function in concert with other factors
- Activation of traA and traC promoters by TraR
- The tra box sequence
- Repression of traA and traC promoters
- Summary
Topics Covered
- Agrobacterium and tumor inducing plasmid
- T-DNA transfer
- Using Agrobacterium to create "transgenic" plants
- VirA and VirG
- Two component system
- Quorum sensing
- LuxR-LuxI-like regulatory system composed of TraR and TraI
- Acylhomoserine lactones (AHLs)
- The crystal structure of TraR
- TraR and expression of target promoters
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Talk Citation
Winans, S. (2008, January 28). Agrobacterium tumefaciens [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 16, 2024, from https://doi.org/10.69645/LIWM8486.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Stephen Winans has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.