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1. Introduction
2. Scientific process
3. The conserved Wnt/β-catenin signaling pathway
4. The Wnt/β-catenin signaling pathway
5. Wnts regulate the body plan
6. Three stories about the Wnt pathway
7. The awesome power of model organisms to discover pathways regulating development
8. Identification of wingless as a recessive gene
9. Wnts are secreted proteins & signal at close range
10. Wg/Wnt signaling phenotypes
11. Wingless discovery in genetic screens in Drosophila
12. Wg/Wnts regulate planar cell polarity
13. The Wnt/Wingless signaling pathway elucidated by the awesome power of Drosophila genetics
14. Mouse
15. The discovery of Int-1
16. Mouse mammary tumor virus
17. Random insertion of a retrovirus can activate a cellular proto-oncogene
18. MMTV insertion activates a cellular oncogene
19. Int-1 is identical to the Drosophila segment polarity gene wingless
20. Wnt meetings - a great place to share new data
21. A new nomenclature: the Wnt gene family
22. Wnt - a signaling pathway gene that regulates development is also involved in cancer
23. Wingless (Wg) is a secreted morphogen
24. Mammalian Wnt1 is also a morphogen
25. What about Wnt signaling pathways in humans?
26. Even simple animals have many Wnt genes
27. Mutations in Wnts cause developmental defects
28. Mutations in Wnt pathway genes also cause developmental defects
29. Wnts are required for adult bones
30. What all these Wnts do?
31. How do we know about Wnt signaling in human cancers?
32. Colorectal cancer
33. The intestinal adenoma-carcinoma sequence
34. Lessons from hereditary colorectal cancer
35. Using the flexible colonoscope
36. Intestinal polyps are precursors to cancer
37. Familial Adenomatous Polyposis (FAP)
38. Identification of the gene
39. APC mutations are found in FAP
40. Precursor lesions of the colon are readily biopsied
41. Colonoscopy allows analysis of early lesions
42. Mutations accumulation is required for cancer development
43. Truncating mutations of the APC gene
44. But isn’t this a lecture on Wnt signaling?
45. Immunoprecipitation of APC
46. The model
47. Where in the colon do APC mutations happen?
48. The intestinal crypt - a clonal conveyor belt
49. Active Wnt/β-catenin signaling in the intestinal crypt
50. Inhibition of Wnt signaling blocks proliferation
51. Wnts and Wg
52. Break

Topics Covered

• The Wnt/β-catenin signaling pathway
• Wg/Wnt signaling phenotypes
• Wingless discovery in genetic screens in Drosophila
• The discovery of Int-1
• Random insertion of a retrovirus can activate a cellular proto-oncogene
• Wnt: a signaling pathway gene that regulates development is also involved in cancer
• Mutations in Wnts cause developmental defects
• Colorectal cancer
• Familial Adenomatous Polyposis (FAP)

Links

Series:

• The Molecular Basis of Cancer

Categories:

• Cancer
• Cell Biology

Therapeutic Areas:

• Oncology

Talk Citation

Publication History

• Published on September 30, 2024

Financial Disclosures

• There are no commercial/financial matters to disclose.