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Printable Handouts
Navigable Slide Index
- Introduction
- Satellite cells and apoptotic signaling
- TUNEL assay of young and aged SCs
- Satellite cells and apoptosis signaling cascade
- Sirt1 activation increases satellite cell proliferation
- miRNA regulation of apoptotic signaling
- miRNA in apoptosis
- Signal-induced apoptosis prevention
- miR‐434‐3p and mitochondrial membrane potential
- miR‐434‐3p targets eIF5A1
- Induction of apoptosis in C2C12 myotubes
- Interventions in apoptotic signaling
- Interference of apoptotic signaling (1)
- Interference of apoptotic signaling (2)
- Different apoptotic responses in WT and Bax-/-
- Pro/anti apoptotic protein levels: WT vs. Bax-/-
- Endurance exercise affects apoptotic signaling
- Proteins after endurance training (1)
- Proteins after endurance training (2)
- Anti-apoptotic role for exercise – further evidence
- Apoptotic components responsive to exercise (1)
- Apoptotic components responsive to exercise (2)
- Nutritional modulators of apoptosis signaling
- Apoptotic nuclei: control and suspended muscles
- Apoptotic nuclei in control after reloading (1)
- Apoptotic nuclei in control after reloading (2)
- Cleaved caspase-9 levels: control, HS or reloading
- Bax protein abundance: control, HS or reloading
- EGCg improves muscle recovery after disuse
- Sirtuin 1 -regulated apoptosis signaling
- Hormonal modulators of apoptosis signaling
- Hormonally regulated apoptosis in muscle
- Hormonal regulation of apoptosis
- H2O2 increases FOXO3a phosphorylation
- Summary
- Acknowledgements
- Appendix 1: apoptosis and loading
- Appendix 2: apoptosis unloading/disuse
- Appendix 3: apoptosis and denervation
- Appendix 4: apoptosis and aging (1)
- Appendix 4: apoptosis and aging (2)
- Appendix 5: apoptosis and exercise (1)
- Appendix 5: apoptosis and exercise (2)
Topics Covered
- Satellite cells and apoptotic signaling in aging muscle
- MicroRNA regulation of apoptotic signaling in aging muscle
- Interventions in apoptotic signaling (denervation vs. exercise)
- Nutritional & hormonal modulators of apoptosis signaling pathways in muscle wasting in aging
Talk Citation
Alway, S.E. (2018, December 2). Pathways of apoptosis in muscle 2 [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 8, 2024, from https://doi.org/10.69645/LJEH5620.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Stephen E. Alway has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Pathways of apoptosis in muscle 2
Published on December 2, 2018
42 min
A selection of talks on Cell Biology
Transcript
Please wait while the transcript is being prepared...
0:04
Satellite cells and apoptotic signaling in aging muscle.
Satellite cells are normally quiescent nuclei or muscle stem cells,
that sit between the basement membrane and the sarcolemma of a muscle fiber.
When these cells are activated they proliferate,
and are essential to the repair or regeneration of muscle
after injury or in response to even exercise or other loading stimuli.
In aging, satellite cell proliferation is suppressed
as there is differentiation capacity that is limited,
and that means that muscle repair is inhibited.
0:42
Satellite cells and apoptotic signaling, TUNEL labeling,
identifies DNA nicks and are DNA fragments that occur in the nuclei.
It turns out that apoptotic signaling is elevated in
satellite cells and muscles from old subjects as compared to young subjects,
and fully at all.
If isolated satellite cells,
culture them for four to 72 hours,
and then they imaged them after incubation with
the TUNEL substrate to identify apoptotic nuclei.
Fulle reported that there was a marked increase in the TUNEL labeling
as indication of apoptosis from these cells isolated from older subjects,
and they were much higher than them as compared to the younger subjects.
1:30
So, Fulle and co-workers have described more completely
the apoptotic signaling cascade that
occurred in satellite cells from young and old muscles,
from averaging age subjects that were
71 years old and they compared them to subjects that were 27 years old.
In the TUNEL labeling was evaluated as an apoptotic index along with annexin V,
which was another indicator of apoptotic nuclei.
Their data suggest that humans satellite cells are indeed susceptible to a apoptosis.
The interesting and important note,
is that satellite cells are in fact eliminated,
then this will result in a reduction in
the ability for muscles to repair in the aging environment.