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Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Introduction to particle characterization
- Nanotechnology
- Importance and applications
- Nanomaterials and nanomedicine (1)
- Nanomaterials and nanomedicine (2)
- Objectives of this lecture
- Colloids
- Particle size and shape
- ESD vs ABD (1)
- ESD vs ABD (2)
- Radius of gyration & hydrodynamic radius
- Distribution of particles: single parameter
- Representing particle size distributions
- Distribution statistics
- Particle size distribution
- Other mathematical parameters
- Representing data on a cumulative curve
- Particle characterization techniques (1)
- Particle characterization techniques (2)
- Different particle size ranges
- Particle characterization techniques (3)
- Light microscopy
- Light microscopy: coacervate particle
- Light obscuration
- Particle shape affects LO accuracy
- Optical microscopy
- Example: flow imaging microscopy data
- Interaction with light
- Laser diffraction/static light scattering
- Laser beam–particle interaction
- Laser diffraction data
- Dynamic light scattering
- Dynamic light scattering: Stokes-Einstein equation
- DLS data
- Zeta potential (1)
- Zeta potential (2)
- DLS and Zeta measurements
- Coulter counting
- Coulter counting example data
- Sieve analysis
- Advanced microscopy techniques: AFM
- AFM example data
- Advanced microscopy techniques: SEM and TEM
- Recent advances and future trends
- Challenges and considerations
- Conclusions
- Acknowledgements
Topics Covered
- Particle characterization
- Types of particles
- Nanotechnology
- Colloids
- Particle size distribution statistics
- Particle characterization techniques
- Light microscopy
- Optical microscopy
- Light obscuration
- Static Light Scattering (SLS) and Dynamic Light Scattering (DLS)
- Zeta potential
- Coulter counting
- Atomic Force Microscopy (AFM)
- Advanced microscopy techniques
Links
Categories:
External Links
Talk Citation
Balog, E.R.M. (2024, February 29). Particle characterization and size analysis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/AVBM3403.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Eva Rose M. Balog has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Cell Biology
Transcript
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0:00
Hello, my name is
Eva Rose Balog,
and I am an Associate
Professor of
Chemistry at the
University of New England.
Today I will be telling
you about methods
of particle characterization
and sizing.
0:14
In this talk, I'll start by
discussing the significance
and practical applications of
particle characterization.
I'll lay out our
lecture objectives,
focus on the basics
of particles,
dive into types of particles,
and explore size, definitions
and distributions.
We'll survey selected
common techniques
for particle characterization.
While we don't have time to do
a deep dive into each
of these techniques,
please know that each of them is
theoretically and
technically very
interesting and their
physical principles
are very fun to learn about.
But we'll focus more
on applications.
Then we'll consider some
questions and challenges.
Look at recent advances
and future trends.
Finally, we'll wrap up with
some key conclusions
of the lecture.
0:60
Let's start by defining
what we mean by a particle.
One theoretical definition is
a bounded region of space with
distinct physical or
optical properties
compared to its
surrounding medium.
In practical terms, a
particle is a small,
distinguishable entity
within a sample,
something we can isolate,
measure, and characterize
independently.
1:25
In nanotechnology,
bioscience, and
pharmaceutical science,
the particles under
study usually
range from individual
biomolecules at
the nanoscale to
vesicle-like assemblies
or individual cells or
compartments at the
micrometer scale.
People studying natural
particulate materials
like soil or dust,
or air pollution in
the geosciences or
industrial materials
like cement or
advanced rubbers might
deal with larger particles
in the millimeter range.
But this talk will
mostly discuss particles
that are in the microscopic
or subvisible range,
Which includes
nanomaterials like
liposomes and
polymeric assemblies.