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Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- It's a GPCR world
- The superfamily of GPCRs
- The problem…
- Untangling the GPCR signalling wires
- Luteinizing hormone receptor (LHR)
- LHR is a key component of the HPG axis
- HPG axis: knockout mice models
- Sexual development of LHR knockout mice
- The different functional forms of GPCR
- The LHR
- The blind and the lame
- LHR dimerisation
- What about the females?
- LH receptor function in vivo
- Functional complementation
- LHR functional complementation
- What kind of LHR complexes are formed?
- Resolution of different imaging techniques
- Resolving single molecules
- Which single molecule method should I use?
- Photoactivated localization microscopy (PALM)
- PD-PALM
- Quantification of functional GPCR oligomers
- LHRS- and LHRB- as investigative tools
- The LHRS-:LHRB- ratio effect on signal output
- Molecular details of LHR oligomer assembly
- Functionally asymmetric GPCR heteromers
- LHR/FSHR crosstalk
- Visualization of LHR/FSHR heteromers
- Summary
- Programming GPCR signalling via homo- and hetero-oligomers
- Acknowledgements
Topics Covered
- Introduction to G protein-coupled receptors (GPCRs)
- Gonadotrophin hormone receptor (GpHR) function
- Role of GPCR oligomerization in vivo through studies of GpHRs
- Single molecule/Super-resolution imaging
- Super-resolution of imaging of GPCRs and GpHRs via PALM
- Applications of PALM to structure/function analysis of GPCR oligomers
Links
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External Links
Talk Citation
Hanyaloglu, A. (2021, March 30). Super-resolution imaging of GPCR oligomers: applications and functional roles [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 12, 2024, from https://doi.org/10.69645/JREI1432.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no commercial/financial matters to disclose.
Other Talks in the Series: G Protein-Coupled Receptors (GPCRs) Signaling in Health and Disease
Transcript
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0:00
Hello, my name is Aylin Hanyaloglu,
I'm from the Institute of Reproductive and Developmental Biology
at Imperial College London.
My lecture today is about super-resolution imaging of
G-protein coupled receptor oligomers and how we have applied
super-resolution imaging to understand the functional roles of
these GPCR interactions at the cellular and physiological level.
0:24
Here is the outline of my lecture today.
I will briefly introduce the superfamily of
G protein-coupled receptors and specifically a particular GPCR,
the gonadotrophin hormone receptors, that will be the focus of this lecture.
I will then talk about the role of GPCR oligomerization in vivo through our studies of these gonadotrophin hormone receptors.
Then we'll introduce single-molecule and
specifically super-resolution imaging, and a particular
super-resolution imaging technique called PALM, and how we've used this
to study G protein-coupled receptors and gonadotrophin hormone receptors.
Finally I'll then move on to how we've applied PALM in combination with other approaches,
to understand the structure and functional analysis of these GPCR interactions.
1:09
This top statement is a bit of a biased statement,
but what I wanted to get across with this slide is to demonstrate
all the different roles that these super-family receptors play in our body.
I like to think of these GPCRs not as
molecular sensors of an individual cell's environment,
but how we as individuals respond to our environment.
They have important roles when you're happy,
when you're stressed, when you're in pain,
when you're sitting down to your favourite meal, watching a movie,
you're activating different G protein-coupled receptors.
There are lots of stimuli in our environment that we
consume (or are exposed to) that are ligands for these receptors,
including a number of drugs.
It's perhaps not surprising that even very small changes in
the activity of these receptors can result in a number of different human diseases.
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