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- Models of Investigation
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2. The anti-microbial defense of Drosophila: a paradigm for innate immunity
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4. Innate immune sensing and response
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5. Macrophages and systems biology
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6. Leukocyte recruitment in vivo
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8. Eosinophils
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9. Dendritic cells: linking innate to different forms of adaptive immunity
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11. Innate-like lymphocytes 1
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12. Innate-like lymphocytes 2
- Prof. Adrian Hayday
- Recognition and Signaling
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13. Colony stimulating factor-1 regulation of macrophages in development and disease
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14. Fc receptors: linking innate and acquired immunity
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15. Phagocytosis
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16. Clearance of apoptotic cells and the control of inflammation
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17. Signaling by innate immune receptors
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18. Nuclear receptors at the crossroads of inflammation and atherosclerosis
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19. Humoral innate immunity and the acute phase response 1
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20. Humoral innate immunity and the acute phase response 2
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21. Cytokines regulating the innate response
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22. Arginase and nitric oxide
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23. Novel lipid mediators in resolution of inflammation
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25. Cationic peptides in innate immunity
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26. Iron metabolism and innate immunity
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27. Innate recognition of viruses
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28. Type I interferons in innate immunity to viral infections
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29. HIV-1 and immunopathogenesis: innate immunity
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30. Understanding and combating tuberculosis
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32. Innate immunity and malaria
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33. Innate immunity in children
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34. From bench to bedside: evolution of anti-TNFalpha therapy in rheumatoid arthritis
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35. NOD-like receptors in innate immunity and inflammatory disease
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36. Paneth cells in innate immunity and inflammatory bowel disease
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37. Innate immunity in the brain in health and disease
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38. The fate of monocytes in atherosclerosis
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39. Macrophages, a cellular toolbox used by tumors to promote progression and metastasis
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40. Monocyte/macrophages in innate immunity
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41. Innate immunity in C. elegans
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43. NLR genes: infection, inflammation and vaccines
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44. Manipulation of innate immune response: lessons from shigella
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45. Innate immunity of the lung and adaptation to air breathing at birth
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Printable Handouts
Navigable Slide Index
- Introduction
- T cells recognise class I and II MHC molecules
- Dendritic cells activate naive T cells
- Key features of dendritic cells (DC)
- Talk outline
- Class II MHC pathway
- Ii / MHC class assembly and processing
- Class I MHC pathway
- Non-canonical antigen presentation pathways
- Function of non-canonical pathways
- Antigen capture
- DC maturation and antigen capture/presentation
- Endocytosis is initially stimulated by LPS
- Rsk mediates TLR-signalled antigen capture
- Microbial stimuli transiently arrest migration
- Invariant chain processing and DC migration
- Distinct antigen capture pathways
- Antigen processing
- Constructive vs. destructive processing
- How do T cell epitopes survive?
- Reducing proteolysis can improve presentation
- Antigen presentation
- Models for display in immature and mature DC
- MHC II ubiquitination and cell surface expression
- TLR signalling effects on DC antigen presentation
- TLR ligand enhances presentation
- How does cross-presentation actually work?
- Different pathways for cross-presentation
- 'ER-phagosome' and class I loading
- Elements of the ER required for cross-presentation
- TAP and Sec61 effect on cross presentation
- Endosomal TAP is needed for cross-presentation
- Distinct parallel compartments for MHC l and II
- Two other pathways of cross-presentation
- Antigen presentation by DC in vivo
- DC sub-types in lymphoid organs
- CD8+ and CD8- DC and MHC class I or II
- Why are some DC better at cross-presentation?
- Antigen transfer from migratory to resident DC
- Migratory and resident DC involved in T cell priming
- Plasmacytoid DC can present antigens to T cells
- Summary
- Acknowledgements
Topics Covered
- Conventional and non-conventional antigen processing pathways
- Innate stimuli boost antigen capture, processing and presentation
- Destructive antigen processing is minimised in dendritic cells (DC)
- Class II MHC display is regulated by ubiquitination
- Linking antigens and TLR ligands can enhance presentation
- Distinct endocytic compartments and/or distinct dendritic cell sub-sets may host antigen presentation on class I MHC molecules (cross-presentation) and on class II MHC molecules
- Antigen bearing migratory DC and lymph node resident DC can collaborate to induce T cell responses
Talk Citation
Watts, C. (2009, June 30). Antigen processing and presentation: modulation by innate immune signals [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 3, 2024, from https://doi.org/10.69645/FQLZ6313.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Colin Watts has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Antigen processing and presentation: modulation by innate immune signals
A selection of talks on Immunology
Transcript
Please wait while the transcript is being prepared...
0:00
This is a
talk on antigen processing
and presentation, and
in particular how it's
regulated by innate immune signals.
And it's given by Colin Watts
from the University of Dundee.
0:13
Antigen presentation refers to
the display of short process
peptides on so-called MHC,
or major histocompatibility
complex molecules.
Seen here is a class I MHC molecule
with the short 8-residue peptide
found in a peptide binding groove.
Class I MHC molecules generally
have peptides between 8
and 10 amino acid residues.
Whereas class II MHC molecules
display considerably longer
peptides due to a more open
ended peptide-binding groove.
It's the recognition
of these peptide MHC
complexes by the T
cell antigen receptors
that initiates most
immune responses.
We can distinguish the two
classes of MHC molecule
by the types of
peptide they present.
As simply diagrammed here, class
I MHC molecules display peptides
which are derived
from intracellular,
i.e. cytosolic or nuclear proteins,
which as we'll see in a moment,
become loaded in the
endoplasmic reticulum
and transported to
the cell surface where
they're recognized by CD8 T cells.
Usually the outcome
of recognition is
killing, for example, of the
virally infected or a tumor cells.
In contrast, class II MHC molecules
capture and display peptides
derived from exogenous
proteins, i.e. proteins taken up
into the cell by one or
more forms of endocytosis,
for example, bacterial
toxins like tetanus toxin.
Here the endocytic pathway acts
as the site for peptide loading.
And then following transport
to the cell's surface,
the CD4 T cell can perform various
functions, for example can help
a B lymphocyte to differentiate and become
an antibody producing cell.
The two T cells seen in this slide
are so-called effector T cells.
They have previously
been activated and are
primed to perform an effector
function, which was just described.
But these T cells must have already
been activated by a different type
of antigen presenting cell.
And it's clear, and as well
discussed elsewhere in the series,
that dendritic cells are uniquely
able to prime naive T cells.
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