Registration for a live webinar on 'Neuroleptic malignant syndrome' is now open.
See webinar details
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
- Models of Investigation
-
41 min
1. Antifungal innate immunity in C. elegans- Dr. Jonathan Ewbank
-
44 min
2. The anti-microbial defense of Drosophila: a paradigm for innate immunity- Prof. Jules Hoffmann
-
38 min
3. Phagocytosis in the fruit fly, Drosophila melanogaster- Dr. Lynda Stuart
-
62 min
4. Innate immune sensing and response- Prof. Bruce Beutler
-
61 min
5. Macrophages and systems biology- Prof. David Hume
- Cell Types and Recruitment
-
71 min
6. Leukocyte recruitment in vivo- Prof. Paul Kubes
-
51 min
-
42 min
8. Eosinophils- Prof. Tim Williams
-
63 min
9. Dendritic cells: linking innate to different forms of adaptive immunity- Prof. Ralph Steinman
-
25 min
-
61 min
11. Innate-like lymphocytes 1- Prof. Adrian Hayday
-
41 min
12. Innate-like lymphocytes 2- Prof. Adrian Hayday
- Recognition and Signaling
-
50 min
13. Colony stimulating factor-1 regulation of macrophages in development and disease- Prof. E. Richard Stanley
-
42 min
14. Fc receptors: linking innate and acquired immunity- Prof. Ken G C Smith
-
39 min
15. Phagocytosis- Prof. Joel Swanson
-
44 min
16. Clearance of apoptotic cells and the control of inflammation- Prof. Sir John Savill
-
38 min
17. Signaling by innate immune receptors- Prof. Michael Karin
-
41 min
18. Nuclear receptors at the crossroads of inflammation and atherosclerosis- Prof. Christopher Glass
- Modulation of Effector Responses
-
22 min
19. Humoral innate immunity and the acute phase response 1- Prof. Alberto Mantovani
-
42 min
20. Humoral innate immunity and the acute phase response 2- Prof. Alberto Mantovani
-
54 min
21. Cytokines regulating the innate response- Prof. Anne O’Garra
-
39 min
22. Arginase and nitric oxide- Dr. Peter Murray
-
38 min
23. Novel lipid mediators in resolution of inflammation- Prof. Charles Serhan
-
69 min
-
42 min
25. Cationic peptides in innate immunity- Dr. Dawn Bowdish
-
27 min
26. Iron metabolism and innate immunity- Prof. Tomas Ganz
- Pathogen-Host Interactions
-
46 min
27. Innate recognition of viruses- Prof. Caetano Reis e Sousa
-
50 min
28. Type I interferons in innate immunity to viral infections- Prof. Christine Biron
-
56 min
29. HIV-1 and immunopathogenesis: innate immunity- Prof. Luis Montaner
-
53 min
30. Understanding and combating tuberculosis- Prof. David Russell
-
34 min
-
51 min
32. Innate immunity and malaria- Prof. Douglas Golenbock
- Health and Disease
-
39 min
33. Innate immunity in children- Prof. David Speert
-
59 min
34. From bench to bedside: evolution of anti-TNFalpha therapy in rheumatoid arthritis- Prof. Sir Ravinder Maini
-
33 min
35. NOD-like receptors in innate immunity and inflammatory disease- Prof. Gabriel Nunez
-
38 min
36. Paneth cells in innate immunity and inflammatory bowel disease- Prof. Satish Keshav
-
36 min
37. Innate immunity in the brain in health and disease- Prof. V. Hugh Perry
-
54 min
38. The fate of monocytes in atherosclerosis- Prof. Gwendolyn Randolph
-
49 min
39. Macrophages, a cellular toolbox used by tumors to promote progression and metastasis- Prof. Jeffrey Pollard
- Archived Lectures *These may not cover the latest advances in the field
-
73 min
40. Monocyte/macrophages in innate immunity- Prof. Emeritus Siamon Gordon
-
32 min
41. Innate immunity in C. elegans- Dr. Jonathan Ewbank
-
51 min
-
39 min
43. NLR genes: infection, inflammation and vaccines- Prof. Jenny Ting
-
63 min
44. Manipulation of innate immune response: lessons from shigella- Prof. Philippe Sansonetti
-
35 min
45. Innate immunity of the lung and adaptation to air breathing at birth- Prof. Jeffrey Whitsett
Printable Handouts
Navigable Slide Index
- Introduction
- The first attempt of intravital microscopy
- Intravital microscopy
- White cells in cremasteric venule
- The leukocyte recruitment cascade (1)
- Proposed mechanism for induced rolling
- Role of P-selectin in leukocyte rolling
- Selectins
- Beta2 integrins (CD18)
- LFA-1 and Mac-1 function (1)
- LFA-1 and Mac-1 function (2)
- Leukocyte recruitment cascade (2)
- Adhesion of neutrophils is dependent on LFA-1
- In vivo time lapse: leukocyte crawling
- In vivo time lapse in Mac-1 deficient cells
- Crawling is dependent on Mac-1
- In vivo PECAM-labeled junctions
- In vivo confocal microscopy
- WT neutrophils transmigrate at junctions
- Mac-1 deficiency effect on migration
- Junctional migration
- Mac-1 deficiency and neutrophils emigration time
- Leukocyte recruitment cascade (3)
- The liver vascular bed
- Liver vascular bed - control
- Vascular bed - an inflamed liver
- Neutrophils and adhesion molecules in the liver
- A role for adhesion in sinusoids?
- Endothelial adhesion molecule expression in vivo
- Adhesion molecule expression in various organs
- Hyaluronan expression in the liver
- CD44 is a receptor for HA
- CD44 and HA mediate neutrophil adhesion
- CD44 is not important in hepatic venules
- Occlusion of sinusoids
- CD44 and sinusoidal perfusion
- Sinusoidal leukocyte adhesion is reversible
- Reverse adhesion increases sinusoidal perfusion
- Generation of Th1 and TH2 cells
- Con A-induced liver inflammation
- Multiple compartments for leukocyte recruitment
- Vascular adhesion protein (VAP)-1 (1)
- Vascular adhesion protein (VAP)-1 (2)
- Hepatic VAP-1 expression
- Th1 and Th2 lymphocyte adhesion
- Alpha4 integrin mediates rolling and adhesion
- Complete healing ileum
- Clinical response to natalizumab
- Brain preparation
- Control brain
- Inflamed brain
- T cell-subset recruitment to the CNS in EAE
- Mechanisms of Th1 T cell rolling
- Mechanisms of Th2 T cell rolling
- Th1 and Th2 cells bind P-selectin
- Th1 and Th2 cells can bind selectins
- Monitoring of blood vessels by monocytes
- NKT cells in vascular bed of liver from CXCR6 mice
- NKT cells from CXCR6 mice - enlarged video
- The effect of systemic LPS on cell rolling
- Rapid lung infiltration by neutrophils
- Intravital microscopy of the lung
- Leukocyte trafficking within the lung
- The effect of systemic LPS on the lung
- Neutrophils get stuck in the lung capillary
- Which molecules mediate neutrophil recruitment?
- How do neutrophils enter lungs?
- Neutrophils are activated in endotoxemic blood
- Scheme of the effect of LPS
- Both leukocytes and endothelium respond to LPS
- Bone marrow transfer protocol
- Bone marrow transfer experiment
- LPS effect on TLR4 leukocytes
- Systemic LPS and leukocyte activation
- Candidates for neutrophil recruitment
- Acknowledgements
Topics Covered
- Leukocyte recruitmen
- A multi-step recruitment that involves rolling adhesion, crawling to junctions and emigration
- Role of selectins and integrin subunits in this process
- Role for other adhesion molecules in liver, lung and potentially other organs
- Constitutive patrolling of immune cells in various tissues
- Role for sentinel cells such as endothelium, mast cells and macrophage in inducing recruitment into tissues
Talk Citation
Kubes, P. (2009, June 30). Leukocyte recruitment in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 12, 2024, from https://doi.org/10.69645/OQQL3026.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Paul Kubes has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
