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Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- Virus isolation
- Animal hosts
- Growth of virus on embryonated eggs
- Cell culture
- Primary cell culture
- Subculture of primary cultured cells
- Cell strains and cell lines
- Cell transformation
- Comparison of cultured cell types
- Cultured cell morphologies
- Recognition of virus infection in culture
- Cytopathic effect: morphological changes
- Cytopathic effect: measles
- Cytopathic effect: Vaccinia
- Cytopathic effect: inclusion bodies
- Hemabsorption
- Hemabsorption micrograph
- Talk outline: assay of viruses
- Biological assays of viruses
- Plaque formation
- Plaque micrograph
- Plaque assay
- Plaque assay photograph
- Focus formation
- Focus formation photograph
- Endpoint titration
- Physical assays of viruses
- Hemagglutination
- Hemagglutination test
- Hemagglutination test: Influenza virus
- Direct particle count
- EM of direct particle count
- Comparison of quantitative assays
- One step growth experiment
- Example of one step growth experiment
- Talk outline: virus genetics
- Virus genetics principles
- Scope of virus genetics
- Clinical significance of virus genetics
- Mutation in viruses
- Mutant phenotypes
- Host range virus mutant
- Host range subtypes
- Engineered host range mutants
- Temperature sensitivity
- Temperature sensitive mutants
- Example of temperature sensitivity
- Antiviral drugs and virus genetics
- Uses of drug resistance/dependence
- Example of drug resistance
- Example of drug dependence
- Recombination and reassortment
- DNA virus recombination
- RNA virus recombination - copy choice
- Segmented RNA virus reassortment
- Reverse genetics
- Summary and perspectives
Topics Covered
- Virus cultivation
- Virus isolation
- Animal hosts
- Cell culture
- Recognition of virus infection in culture
- CPE: morphological changes
- Hemadsorption
- Biological assay of viruses
- Plaque assay
- Focus assay
- Endpoint method
- Physical assay of viruses
- Hemagglutination
- Direct particle count
- Virus genetics
- Genetic principles
- Mutation
- Engineered host range
- Temperature sensitive mutants
- Antiviral drugs
- Uses of drug resistance/dependence
- Recombination and reassortment
- Reverse genetics
Links
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Talk Citation
Condit, R. (2007, October 1). Principles of virology II [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 12, 2024, from https://doi.org/10.69645/ESMQ9897.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Richard Condit has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.