Genes in skin wound healing: microRNAs 2

Published on October 7, 2014   33 min
Now I'd like to switch gears and address another novel concept that has originated based on work in our laboratory. The basic premise here is that some elements of inducible wound healing is actually retained in the adult skin tissue in a silenced form. This silencing is primarily executed by microRNA. As one example, we have discovered that miR-200b in skin, microvascular endothelial cells, act as an angiostatic agent during wounding. This silencer is desilenced, enabling wound angiogenesis to start. Let's look at the data here. In Panel A, you see human microvascular dermal endothelial cells, a scratch assay has been done to study the migration of these cells. And if you added miR-200b mimic to these cells you clearly blunt or decelerate the migration of the cells across the scratch. In Panel B, it is shown that the ability of these cells to from tubes in a matrigel, which is a sign of angiogenic property, such angiogenic property is remarkably blunted or impaired in the presence of miR-200b mimic. Supporting the notion that miR-200b has potent angiostatic properties.