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Printable Handouts
Navigable Slide Index
- Introduction
- miR-200b: potent angiostatic miRNA
- Down-regulation of miR-200b in cutaneous wound
- miR-200b induction inhibits wound angiogenesis
- Diabetic wounds: multiple factors
- Impaired closure & angiogenesis in diabetic mice
- Diabetic wounds and miR-200b expression
- Lack of GATA2 and VEGFR2 induction
- miR-200b induces translational repression
- Importance of skin miRNA function for healing
- Biofilms: microbial social network
- Multi-species biofilm infection - swine model (1)
- Multi-species biofilm infection - swine model (2)
- Characterization of porcine burn wound biofilms
- Biofilm specific gene expression in bacteria
- Debridement is ineffective with burn wound biofilms
- Biofilm compromises skin barrier function
- Biofilm impairs tight junction function
- Focal silencing of ZO-1 and ZO-2 by biofilm
- Biofilm induces specific skin miRNA
- miR-146a and miR-106b target ZO-1 and ZO-2
- Topical delivery of miR-146a and miR-106b
- Concluding remarks
- Acknowledgements
Topics Covered
- miR-200b: potent angiostatic miRNA
- Diabetic wounds
- Wound closure and angiogenesis in diabetic wounds
- miR-200b expression in diabetic wounds
- GATA2 & VEGFR2
- Biofilms: microbial social network
- Multi-species biofilm infection in a swine burn wound model
- Biofilm specific genes expressed in bacteria
- Biofilm impairs tight junction function
- Silencing of ZO-1 and ZO-2 by biofilms
- Biofilm induces specific skin miRNA:miR-146a & miR-106b
Talk Citation
Sen, C.K. (2014, October 7). Genes in skin wound healing: microRNAs 2 [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 11, 2024, from https://doi.org/10.69645/QYUG3188.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Chandan K. Sen has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Genes in skin wound healing: microRNAs 2
Published on October 7, 2014
33 min
A selection of talks on Genetics & Epigenetics
Transcript
Please wait while the transcript is being prepared...
0:04
Now I'd like to switch gears and
address another novel concept that
has originated based on
work in our laboratory.
The basic premise here is that some
elements of inducible wound healing
is actually retained in the adult
skin tissue in a silenced form.
This silencing is primarily
executed by microRNA.
As one example, we have
discovered that miR-200b in skin,
microvascular endothelial cells,
act as an angiostatic agent
during wounding.
This silencer is
desilenced, enabling
wound angiogenesis to start.
Let's look at the data here.
In Panel A, you see human
microvascular dermal endothelial
cells, a scratch assay
has been done to study
the migration of these cells.
And if you added miR-200b
mimic to these cells
you clearly blunt or decelerate
the migration of the cells
across the scratch.
In Panel B, it is shown that
the ability of these cells
to from tubes in a matrigel, which
is a sign of angiogenic property,
such angiogenic property is
remarkably blunted or impaired
in the presence of miR-200b mimic.
Supporting the notion that miR-200b
has potent angiostatic properties.