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Printable Handouts
Navigable Slide Index
- Introduction
- Threats from pathogens
- Strategies to treat infections
- Antibiotic resistance
- E. coli MarR
- Bacterial multidrug resistance regulators
- MarR family transcriptional factors in S. aureus
- Susceptibility of S. aureus mutants to NOR
- Resistance to quinolone for S. aureus strains
- Virulence test using the murine abscess model
- Crystal structure of MgrA
- Molecular mechanism for MgrA-based regulation
- A unique Cys residue
- Oxidation sensing?
- Cysteine sulfenic acid formation in MgrA
- Gel-shift assay to study MgrA-DNA interaction
- Bio-relevance: plate sensitivity assays
- Further in vivo results
- MgrA is a redox switch in S. aureus
- SarZ, an MgrA homologue in S. aureus
- SarZ is also an oxidation sensor
- SarZ plays a complementary role next to mgrA
- Crystal structure of the reduced SarZ
- Crystal structure of oxidized SarZ with Cys13-SOH
- SarZ and mgrA are major ROS in S.aureus
- How S. aureus senses host immune defense
- High virulence/low virulence hypothesis
- Virulence suppression strategies
- PVS works like hydrogen peroxide
- An epoxide as a mild and selective alkylator
- Phenyl epoxide sulfonate (PES) activates MgrA
- The in vivo effect of the epoxide
- Developing small molecule modulators
- FA-based HTS for non-covalent modulators
- NSRB at Harvard
- MgrA homologues in other pathogens
- Two MgrA homologues in P. aeruginosa
- OspR in P. aeruginosa
- Cys24 in OspR is redox active
- OspR plays a role in virulence
- P. aeruginosa MexR
- MexR involved in regulating resistance: hypothesis
- MexR oxidation leads to its dissociation from DNA
- Intermonomer disulfide bond formation in MexR
- Computational analysis of oxidized MexR structure
- Redox potential measurement of MexR
- Experiments probing in vivo response
- MexR senses peroxide stress
- Crystal structure of the oxidized MexR
- Conclusion
- Acknowledgements
Topics Covered
- Virulence regulation
- oxidative stress from immune response can dramatically affect the virulence in certain human pathogens-antibiotic resistance-oxidative stress induced by antibiotic treatment can affect resistance-Staphyloccocus aureus-Pseudomonas aeruginosa
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Talk Citation
He, C. (2011, March 15). Virulence and antibiotic resistance regulation in pathogens [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 12, 2024, from https://doi.org/10.69645/HSDF1712.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Chuan He has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.