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Printable Handouts
Navigable Slide Index
- Introduction
- Sanger sequencing
- Sanger limitations
- The Archon Genomics Xprize
- Sequencing by synthesis
- 454 sequencing by Roche
- Sample preparation: DNA fragmentation
- Addition of adaptors
- Adaptors A and B
- Isolation of fragments with only one A and one B
- Binding of the DNA fragments to capture beads
- Formation of 'micro-reactors'
- Clonal amplification step
- Addition of the sequencing primer
- Loading the beads onto a PicoTiter plate
- Sequencing reaction
- Illustration of the sequencing reaction
- Flowgram
- Assembly of the entire target sequence
- Solexa sequencing by Illumina
- Sample preparation for Solexa sequencing
- Fixation of the strands onto a flow cell
- Formation of a DNA fragment bridge
- Creation of a double-stranded bridge by PCR
- Formation of single-stranded DNA array
- Clonal amplification of DNA clusters
- Formation of the sequencing templates
- Reversible terminators
- The sequencing reaction of Solexa technology (1)
- Laser excitation
- The sequencing reaction of Solexa technology (2)
- Analysing flow cell images
- End result of Solexa sequencing
- SOLid sequencing by ABI
- Sample preparation for SOLid sequencing
- Deposition of the DNA beads onto glass slide
- Pre-sequencing step of SOLid technology
- The probes (1)
- The probes (2)
- The sequencing reaction of SOLid technology
- Detecting a single colour does not indicate a base
- Colour code gives multiple possibilities
- Decoding the code using base-pair "0" (1)
- Decoding the code using base-pair "0" (2)
- End result of SOLid sequencing
- Stats for the 3 main competitors
- Some other methods
- True single molecule sequencing
- Sequencing by hybridization
- Nanopore sequencing
- Real time polymerase sequencing
- Further reading
Topics Covered
- Sanger sequencing
- The Archon Genomics Xprize
- Sequencing by synthesis 454 sequencing by Roche
- Solexa sequencing by Illumina
- SOLid sequencing by ABI
- Stats for the 3 main competitors
- Some other methods
- True single molecule sequencing
- Sequencing by hybridization
- Nanopore sequencing
- Real time polymerase sequencing
Talk Citation
Veeramah, K. (2009, December 30). Next generation sequencing technologies [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/YBQL4732.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Krishna Veeramah has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.