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- Principles in Bacterial Pathogenesis
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43 min
1. The molecular basis of bacterial pathogenicity: an overview- Prof. B. Brett Finlay
- Gram Negative Pathogens
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50 min
2. Deciphering shigella invasion of epithelial cells- Prof. Philippe Sansonetti
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43 min
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57 min
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34 min
5. Bordetella pertussis- Prof. Alison Weiss
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37 min
6. Salmonellae: molecular basis of infection- Prof. Samuel Miller
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43 min
7. The diversity of Escherichia coli infections- Prof. Michael Donnenberg
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18 min
8. Bacterial activation of epithelial signaling- Prof. Alice Prince
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48 min
9. Human pathogenic Yersinia species- Prof. James Bliska
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32 min
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46 min
11. Dental pathogens- Prof. Ann Progulske-Fox
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35 min
12. Haemophilus- Prof. Robert Munson
- Gram Positive Pathogens
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53 min
13. The bacterial pathogen Listeria monocytogenes: an amazing multifaceted model- Prof. Pascale Cossart
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42 min
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48 min
15. Molecular pathogenesis and prevention of Staphylococcus aureus infections- Prof. Olaf Schneewind
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49 min
16. Streptococcus pyogenes disease and molecular pathogenesis- Prof. P. Patrick Cleary
- Host Responses
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29 min
17. Microbial recognition and the immune response- Dr. Dana Philpott
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40 min
18. Enteric pathogens-microbiota-host inter-kingdom chemical interactions- Prof. Vanessa Sperandio
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47 min
19. Toll-like receptor signalling during infection and inflammation- Prof. Luke O'Neill
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56 min
20. The human indigenous microbiota- Prof. David Relman
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53 min
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47 min
22. Role of neutrophils in acute lung injury and repair- Prof. Gregory Downey
- Preventatives and Therapeutics
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35 min
23. Antibiotics and antibiotic resistance- Prof. Gerry Wright
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36 min
24. Vaccines: a health insurance of the 21st century- Prof. Rino Rappuoli
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41 min
25. Biodefense vaccines- Prof. James Nataro
- Archived Lectures *These may not cover the latest advances in the field
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39 min
26. Vaccines in the modern world- Prof. Gordon Dougan
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39 min
27. Bacterial infection of epithelial signaling- Prof. Alice Prince
Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Salmonellae: ubiquitous pathogens
- Human diseases associated with Salmonellae
- Species specificity and disease
- Gastrointestinal infections
- Risk factors: gastrointestinal infections
- Bacterial foodborne diseases in the US
- Salmonellosis outbreaks linked to alfalfa sprouts
- Recent Salmonella outbreaks
- Salmonella St. Paul outbreak 2008
- Salmonella pathogenesis
- Salmonella colonize Peyer's patches
- How does Salmonella manipulate host cells?
- Type III secretion system (TTSS)
- Needle complex assembly pathway
- Salmonella encode two TTSS
- SPI1 and SPI2 translocation
- SPI1 mediates enteropathogenesis
- Salmonella enter cells by macropinocytosis
- SPI-1 effectors modify actin structure
- Small GTPase activation
- SPI induced effectors
- Inflammation is induced by signaling
- TLR's and NLR's induced inflammation
- How does Salmonella survive in the phagosome?
- How bacteria resist innate immunity and replicate
- The PhoP/PhoQ system is required for virulence
- The PhoPQ two-component system
- PhoP/PhoQ system is induced in phagosomes
- The PhoP/PhoQ signals
- Structural classes of antimicrobial peptides
- PhoQ has a PAS domain, not a binding pocket
- PhoQ has a PAS Domain with novel alpha helices
- Ca++ bound state of the PhoQ sensor domain
- Metal mediates PhoQ contact with the membrane
- Antimicrobial peptide and metal binding sites
- A model for signaling
- Sensing acidic pH by a different mechanism
- pH affects the conformation of the PhoQ sensor
- H157R mutation stabilizes low pH conformation
- Rigidity in acidic patch promotes repressed state
- Network destabilization increased helix movement
- The PhoQ periplasmic domain
- Remodel bacterial surface
- Bacterial envelope remodeling
- PhoPQ represses synthesis of organelles
- Antimicrobial peptide resistance genes
- Salmonella typhimurium LPS modifications
- Modifications associated with peptide resistance
- Bacterial resistance mechanisms to peptides
- Modify host processes
- SPI1 and SPI2 TTSS are expressed temporally
- Phagosome localization and morphological change
- Salmonellae phagosome tubulation
- Possible functions of phagosome tubulation
- What are phagosome tubulations?
- What is the role of SifA?
- A family that activates small GTPase pathways
- SifA-SKIP complex demonstrates two domains
- The SifA-SKIP complex showing the WxxE motif
- SifA-C terminus: similar to Salmonella GEF SopE
- SifA alone does not induce endosomal tubulation
- SifA and SseJ induce endosomal tubulation
- SseJ is similar to mammalian GCAT proteins
- SseJ enzymatic activity is required for tubulation
- Is RhoA the SifA target?
- RhoA induces SseJ-coated membranes extension
- Other GTPases do not induce tubulation with SseJ
- SseJ binds RhoA
- SifA binds to GDP-bound RhoA
- A possible complex SKIP/SifA/RhoA/GDP SseJ
- Salmonella induced phagosome tubulation model
- Summary
Topics Covered
- Salmonellae
- Gram-negative bacteria that cause gastroenteritis and systemic disease or enteric fever
- Mechanisms of bacterial pathogenesis include: sensing host environments through a specific receptor, remodeling the bacterial surface, and altering host cells processes
- A receptor that recognizes antimicrobial peptides and low pH
- Regulation of the structure of lipopolysaccharide, and alteration of the Salmonella-containing phagosome by use of a specialized secretion system that functions to accomplish protein transport to the mammalian cell cytoplasm
Links
Series:
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Talk Citation
Miller, S. (2009, October 29). Salmonellae: molecular basis of infection [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2025, from https://doi.org/10.69645/KSRE9463.Export Citation (RIS)
Publication History
- Published on October 29, 2009
Financial Disclosures
- Prof. Samuel Miller has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Salmonellae: molecular basis of infection
Published on October 29, 2009
37 min
