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1. Introduction
2. Legionella pneumophila
3. The Legionella lifecycle
4. The dot and icm genes
5. dot/Icm define type 4 secretory system
6. Dot/Icm and intracellular replication
7. Endocytic membrane transport
8. Dot/Icm effect on endocytic maturation
9. Understanding the function of Dot/Icm
10. Dot/Icm and transport of Legionella vacuole
11. Legionella subverts vesicular transport
12. Legionella replication requires vesicular transport
13. ARF function is required for ER remodeling
14. ER-derived vesicles and Legionella vacuole
15. Host GTPases are used for replication
16. The Dot/Icm system modulates Arf
17. The Dot/Icm system modulates Rab1
18. Control of Arf and Rab GTPases
19. Identifying Legionella effector proteins
20. Identification of ralF
21. RalF is translocated to the bacterial cell
22. Dot/Icm delivers RalF to the host cell
23. RalF defines a new bacterial member of Sec7
24. Activation of Arf by Legionella
25. How does Legionella modulate Rab1 function?
26. Identification of DrrA
27. DrrA functions as a GEF factor
28. Other Legionella factors that target Rab1
29. DrrA is sufficient for Rab1 recruitment
30. DrrA is able to dissociate GDI from Rab1
31. DrrA has GDI displacement activity
32. DrrA has 2 functions
33. LepB interacts specifically with Rab1-GTP (1)
34. LepB interacts specifically with Rab1-GTP (2)
35. LepB is a Rab1 GAP
36. Control of Rab1 membrane cycling
37. Legionella: the greatest cell biologist on earth

Topics Covered

• The legionella life cycle
• Identification of the dot and icm genes
• The dot and icm genes define a new type IV secretion system
• Intracellular replication
• Endocytic membrane transport
• Transport of the Legionella-containing vacuole
• Legionella replication
• The dot/icm system modulates Arf and Rab1
• Can we identify Legionella proteins that modulate Arf and Rab1 function?
• Activation of Arf by Legionella
• Can Legionella modulate the function of other host GTPases?
• Identification of DrrA
• LepB
• Legionella: The greatest cell biologist on Earth

Links

Series:

• Molecular Basis of Bacterial Infection

Categories:

• Diseases, Disorders & Treatments
• Immunology
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on October 29, 2009

Financial Disclosures

• Dr. Craig Roy has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.