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Printable Handouts
Navigable Slide Index
- Introduction
- Protein misfolding is a significant clinical problem
- Heat shock proteins protect cells from stress
- Cellular processes involving Hsp70
- Regulated reaction cycle controls Hsp70 function
- Yeast prions
- Sup35 solubility determines suppressor phenotype
- Chaperone mutations impair amyloid propagation
- Chaperones activity and prions "seed" replication
- L483W mutation of Hsp70
- Location of mutations on Hsp70 structure
- Altered substrate binding and amyloid propagation
- Fes1 affects [PSI+] as anticipated
- Hsp70 co-chaperones and amyloid propagation
- Sup35 prion domain-GFP fusion protein
- Aggregates of Sup35 are larger in Hsp70 mutant
- Larger polymers or larger aggregates of polymers?
- Size of Sup35 polymers in Hsp70 mutant
- Activity of mutant Hsp70
- Cytosolic Hsp70 Ssa isoforms
- Hsp70 isoforms influence prions differently
- Differences between Ssa1 and Ssa2
- Hsp104 resolubilizes proteins from aggregates
- Guandine-hydrochloride "cures" yeast of prions
- Kinetics of guanidine curing of [PSI+]
- Hsp104 fragments prion polymers
- Hsp104 makes new prion "seeds"
- Threading activity correlates with [PSI+] strength
- Amyloid breaking by Hsp104/70/40 machinery
- [PSI+] prion requiring modestly elevated Hsp104
- Effects of elevated Hsp104 on [PSI+]
- Ssa1 antagonizes curing of [PSI+] by Hsp104
- NTD required for excess Hsp104 to cure [PSI+]
- Effects of altered chaperone expression on prions
- Chaperone involvement in prion replication
Topics Covered
- Role of protein chaperones in protein folding
- Yeast prions as amyloid forms of cellular proteins
- Requirement of chaperone machinery for prion propagation
- Chaperone mutants adversely affect prions and provide insight into chaperone/amyloid interactions
Talk Citation
Masison, D. (2008, September 4). Yeast prions and protein chaperones [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 14, 2024, from https://doi.org/10.69645/RLVI5934.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Daniel Masison has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.