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Printable Handouts
Navigable Slide Index
- Introduction
- DNA plasmids
- The yeast plasmid
- Plasmid amplification
- Fluorescence-tagged 2 micron plasmid
- Plasmid dynamics during the cell cycle
- Chromosome and plasmid segregation
- Cell cycle and cohesin-STB association
- Cohesin cleavage and plasmid partitioning
- CSE4-STB association
- Cohesin-STB association in the CSE4 mutant
- Protein association at STB at G1-S
- Pathway for equal plasmid segregation
- Cohesion in the 2 micron plasmid
- Plasmid pairing is cohesin-mediated
- Cohesin-mediated sister-to-sister counting
- Random grouping of replicated plasmids
- STB-STB segregation
- CEN-CEN segregation
- CEN-STB segregation
- Summary
Topics Covered
- The yeast plasmid as a highly optimized design for a multi-copy selfish DNA element
- The need for an active partitioning system for the segregation of a high copy plasmid
- Coupling between plasmid segregation and chromosome segregation
- Poaching of host factors required for chromosome segregation by the plasmid partitioning system
- Cohesin mediated equal segregation of duplicated plasmid clusters
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Talk Citation
Jayaram, M. (2008, January 28). The yeast plasmid: a highly persistent selfish DNA element [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/LITN8042.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Makkuni Jayaram has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.