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Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- What are IS elements?
- Why are IS elements interesting?
- Discovery of insertion sequences (IS elements)
- Transposition
- IS structure
- Gamma-delta and Tn4
- IS elements can form composite transposons
- Composite transposon formation
- Cut and paste vs. replicative transposition
- Mechanism of cut and paste IS transposition
- IS50-like transposition
- Crystal structure of the IS50 synaptic complex
- Transposase proteins
- Regulation of IS transposition
- Role of IS elements in bacterial genetics
- Schematic of the F plasmid
- IS-mediated integration of F to generate HFR
- IS-mediated F' formation
- F' formation by deletion formation
- R factors
- Conclusion
Topics Covered
- Insertions sequences
- A major form of mobile genetic elements in eubacteria and archaebacteria
- Insertion sequences move via DNA transposition
- Insertion sequences cause genome changes (insertions, deletions, inversions and chromosome fusions) and facilitate the horizontal transfer of genes
- Related processes include integration of HIV-1 DNA
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Talk Citation
Reznikoff, W. (2008, January 28). Insertion sequences and DNA transposition [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/AIRB5151.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. William Reznikoff has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.