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1. Where the thoughts dwell: history of neuroscience and introduction into neuroglia
- Prof. Alexei Verkhratsky
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2. Astrocytes: definition, appearance and general physiology 1
- Prof. Alexei Verkhratsky
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3. Astrocytes: definition, appearance and general physiology 2
- Prof. Alexei Verkhratsky
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4. Glial calcium signalling
- Prof. Alexei Verkhratsky
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5. Astroglial calcium signalling: what it could tell us
- Prof. Dmitri Rusakov
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6. Neuronal-glial chemical transmission mediated by glutamate and ATP
- Prof. Alexei Verkhratsky
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7. Energy and amino acid neurotransmitter metabolism in astrocytes
- Prof. Helle Waagepetersen
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8. Mechanisms of glutamate release from astrocytes
- Prof. Vladimir Parpura
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9. Cannabinoid type 1 receptors in astrocytes
- Prof. Giovanni Marsicano
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10. Neuronal-glial anatomical plasticity in the hypothalamus
- Dr. Stéphane H.R. Oliet
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11. General physiology of oligodendrocytes
- Prof. Arthur M. Butt
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12. Neuropathology of microglia and oligodendrocytes
- Prof. Arthur M. Butt
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13. Oligodendroglia/MCT1 are an unexpected contributor to CNS toxicity/neurodegeneration
- Prof. Jeffrey D. Rothstein
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14. Physiology and pathophysiology of microglia
- Prof. Dr. Uwe-Karsten Hanisch
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15. Properties of regulated exocytosis in astrocytes
- Prof. Robert Zorec
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16. Exocytosis and gliocrine astroglia
- Prof. Robert Zorec
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17. Noradrenergic hypothesis of neurodegeneration and astroglia
- Prof. Robert Zorec
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18. Principles of astrogliopathology: from reactivity to atrophy and degeneration
- Prof. Alexei Verkhratsky
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19. Regulation of synaptic plasticity by astroglia
- Prof. Dmitri Rusakov
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20. Enteric glia: the glue of the enteric nervous system
- Dr. Keith A. Sharkey
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21. Purinergic neuron-to-glia signaling in the enteric nervous system
- Dr. Brian D. Gulbransen
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24. The impact of astrocyte mitochondrial ATP production on neuroprotection after stroke
- Prof. James Lechleiter
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25. Hepatic encephalopathy in cirrhosis: a disorder of glial-neuronal signalling
- Prof. Roger F. Butterworth
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26. Hepatic encephalopathy in acute liver failure: a primary neurogliopathy
- Prof. Roger F. Butterworth
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27. Bi-directional communication at the neurovascular unit: implications for neuronal function
- Prof. Jessica A. Filosa
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28. Astrocyte reactivity
- Prof. Michael Sofroniew
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29. Roles of microglia in the healthy brain
- Dr. Marie-Ève Tremblay
- Archived Lectures *These may not cover the latest advances in the field
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30. Astroglia in neuropathology
- Prof. Alexei Verkhratsky
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31. Cell biology and physiology of astroglia
- Prof. Alexei Verkhratsky
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32. General physiology of oligodendrocytes and Schwann cells
- Prof. Arthur M. Butt
Printable Handouts
Navigable Slide Index
- Introduction
- Follow up of a previous lecture
- Messages
- Overview
- What is exocytosis?
- Eukaryotes
- Inventions
- Endolysosome
- Lysosome
- Development of metazoans
- Quantal theory of neurotransmitter release
- Regulated exocytosis in astrocytes
- Electrophysiology & photolysis of caged calcium (1)
- Electrophysiology & photolysis of caged calcium (2)
- Electrophysiology & photolysis of caged calcium (3)
- Changing calcium in astrocytes
- Ca2+ dependence of the rate of exocytosis in various secretory cells
- Ca2+-dependent exocytosis in astrocytes (1)
- Regulated exocytosis requires the SNARE proteins
- Ca2+-dependent exocytosis in astrocytes (2)
- Microscopy studies
- Gliocrine astrocytes release a myriad of gliosignals via vesicles
- Vesicles and exocytosis
- Monitoring single vesicle fusion (discrete changes in Cm)
- Single-vesicle studies in astrocytes
- Two vesicle size populations in astrocytes
- Fusion pore conductance depends on vesicle diameter
- Vesicle cholesterol
- SNARE complex and vesicle cholesterol affect the transitions from reversible to full fusion
- SNAP-23 replaces SNAP-25 in astrocytes
- Ca2+-dependent exocytosis in astrocytes (3)
- Vesicle mobility
- Directional and non-directional mobility
- Directional mobility requires cytoskeletal elements
- Peptidergic vesicles
- Anti-VGLUT1 vesicle mobility in astrocytes
- Purinergic vesicles
- Reactive astrocytes
- Vesicle mobility & cytoskeletal filaments
- IFs and vesicle mobility
- Reactive astrogliosis in vitro
- Endolysosomal vesicle mobility
- Vesicle membrane interactions
- Summary
- Antigen presentation
- Reactive astrocytes in vitro
- IFNγ and reversible fusion of vesicles
- Mobility of MHC-II vesicles and intermediate filaments
- Conclusions
- Acknowledgements
Topics Covered
- Exocytosis
- Calcium-dependent exocytosis in astrocytes
- SNARE proteins
- Gliocrine astrocytes
- Vesicle fusion
- Vesicle cholesterol
- Vesicle mobility
- Peptidergic vesicles
- Purinergic vesicles
- Reactive astrocytes
- Reactive astrogliosis in vitro
- Vesicle membrane interactions
Links
Series:
Categories:
Therapeutic Areas:
External Links
Talk Citation
Zorec, R. (2023, September 28). Exocytosis and gliocrine astroglia [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 27, 2024, from https://hstalks.com/bs/5413/.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no commercial/financial disclosures.
A selection of talks on Cell Biology
Transcript
Please wait while the transcript is being prepared...
0:00
My name is Robert Zorec.
I'm from Ljubljana.
Today I will talk about
exocytosis in astrocytes.
0:11
I would like to
highlight that this is a
follow up of a previous lecture
on the properties
of exocytosis in
astrocytes that was
recorded in 2014.
Let me tell you that
our lab is studying
pathophysiology at the
subcellular level,
including vesicles.
That actually brings
us to the topic
that, in fact, our
aim is to translate.
So, we want to gain
knowledge of studying
membrane fusion and
vesicle dynamics,
and then the gain results we
hope to be employed in
translating this to medicines.
In the field of cancer,
we have already developed
a lysosomal heterologous
fusion cell-based
immunotherapy to treat cancer.
This therapy is already
available and it is
approved in Slovenia,
and this was done after
following a clinical trial.
In the domain of
neurological indications,
we would like to understand
vesicle-based signaling and
metabolism in astrocytes,
which is then a platform
from which we'd like to
develop small molecules that
target neuroglial aerobic
glycolosis to be used in
treating various disorders,
including neurodegeneration
and neurodevelopment.
This is the foreground
for the lecture today.
1:28
The first message is
that like in neurons,
calcium-dependent exocytosis
exists in astrocytes,
it is controlled by SNARE
proteins and cholesterol,
but is very very slow.
Astrocytes act as
signal integrators and
adaptation to regulate
homeostatic processes
in a slow time domain,
as is the case in the
endocrine system,
brings us to the term:
the gliocrine system.
So, astrocytes are part of
this very slow
regulating system,
hence called the
gliocrine system.
Astrocytes secrete
neurotransmitters and
a wide array of neuromodulators,
hormones, metabolic, trophic,
and plastic factors.
The release of these
gliosignals from astrocytes
occurs through distinct pathways
that include diffusion,
plasmalemmal channels,
translocation by
multiple transporters and
vesicle-based
regulated exocytosis.
That is the topic of
our lecture today.
These vesicles in astrocytes
are of different sizes.
At the end, I'd like
to highlight that
lysosomal vesicle traffic is
altered in reactive astrocytes.