Printable Handouts

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Navigable Slide Index

1. Introduction
2. The history of recombinant antibodies
3. The first approved _x000B_therapeutic antibody
4. Rodent, chimeric and humanized antibodies
5. The first chimeric antibody
6. The first chimeric full length antibody
7. The first humanized antibody
8. The first fully human antibody
9. The road to fully human antibodies
10. Antibody nomenclature
11. Human antibodies take the lead
12. Approved antibodies (10/2018)
13. Antibody phage display - technology
14. The idea of antibody phage display
15. Filamentous phage
16. Replication cycle of M13 phage
17. Antibody structure
18. Antibody phage display
19. Antibody phage display - scFv
20. Antibody phage display - Fab
21. Phagemid vectors
22. Antibody gene libraries
23. Antibody library generation
24. Naive vs. (semi-) synthetic libraries
25. Naive libraries vs. transgenic mice
26. Germinality index
27. Antibody selection (panning)
28. Polyvalent display
29. Antibody phage display - case studies
30. Antibodies to soluble protein antigens
31. Antibodies to soluble protein antigens (graph)
32. Human/mouse cross-reactivity
33. Antibody optimization by shuffling
34. Cross-reactivity (2 step approach)
35. Cross reactivity (2 step approach example)
36. Antibody optimization by shuffling (example)
37. Antibody phage display - difficult targets
38. Cell panning on stably transfected cells
39. Panning on stably transfected cells (workflow)
40. Panning on stably transfected cells (details)
41. Cell panning on transiently transfected cells
42. Transiently transfected cells (workflow)
43. Transiently transfected cells (workflow & data)
44. Phage display discovery pipeline

Topics Covered

• The history of recombinant antibody development
• Road to fully human antibodies
• Antibody phage display technology
• Phage display libraries and generation
• Antibody discovery by phage display
• Antibody optimization by light chain shuffling
• Antibody discovery to difficult targets
• Introduction of a full phage display discovery pipeline

Links

Categories:

• Immunology
• Methods
• Pharmaceutical Sciences

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Frenzel, A. (2019, May 30). Phage display for generating monoclonal antibodies [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 18, 2019, from https://hstalks.com/bs/3942/.

Publication History

• Published on May 30, 2019

Financial Disclosures

• Dr. Andre Frenzel has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.