We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Membranes for manufacturing biotechnology products
- Filter devices
- Membrane chemistry
- Membrane pore size distribution and morphology
- How sterilizing-membrane filter work
- Key characteristics for designing sterile filtration process
- Operation modes: constant pressure vs constant flowrate (flux)
- Classical membrane fouling models
- Fouling analyses
- Fouling analyses method 1: governing equations for flux decline
- Fouling analyses method 2: derivative method
- Sterile filtration process development
- Sterile filtration in biotech manufacturing process
- Summary
Topics Covered
- Microfiltration membranes and devices
- Pore size distribution and morphology of membranes
- Sterile filtration and its operation modes
- Fouling models and analyses
- Filter sizing
- Sterile filtration in biotech manufacturing process
Links
Categories:
External Links
- Giglia et al. J Membrane Sci. 2010 Dec;365(1-2):347-355
- SUPRAdisc™ II Depth Filter Modules
- Liu et al. Environment International. 2019 Oct;131
- Goldrick et al. J Membrane Sci. 2017 Jun;531:138-147
- Ho and Zydney. J Colloid Interf Sci. 2000 Dec;232(2):389-399
- van Reis and Zydney. J Membrane Sci. 2007 Jul;297(1-2):16-50
- Defining a strategy for the Validation and Qualification of Sterile Filtration Processes of Investigational Medicinal Compounds
Talk Citation
Motevalian, P. (2020, February 27). Microfiltration in biotechnology: characterisation and scale up [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 5, 2024, from https://doi.org/10.69645/SXGL6356.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no commercial/financial matters to disclose.
A selection of talks on Methods
Transcript
Please wait while the transcript is being prepared...
0:00
I am Pouria Motevalian and I am scientist
in Bioprocess Research and Development in Pfizer.
Today, we will talk about characterization and scale up
of microfiltration processes for biotechnology application.
0:16
Here is the outline for today's talk.
First, we review the background on application of
membrane filtration for manufacturing biotechnology products,
then we focus on microfiltration and
review microfiltration devices and membrane chemistries.
Next, our focus shifts toward a sterile filtration as one of
the most commonly used form of
microfiltration and review sterile filtration membrane fouling.
Then we talk about the scale up of a sterile filtration processes
and membrane sizing for a sterile filtration application and finish with a summary.
0:58
Membranes are extensively used in production,
purification, and formulation of biotechnology products.
From size and application perspective,
membrane filtration can be categorized into four categories: microfiltration,
ultrafiltration, virus filtration, and nanofiltration.
Microfiltration membranes typically retain bacteria, cell debris,
and intact cells by passing colloids,
viruses, proteins, and salts.
The approximate pore size for microfiltration membranes spans from 50 nanometer
to 10 micron which makes them suitable for multiple upstream and downstream applications.
Upstream applications of microfiltration
include a sterile filtration of fermentation media,
pH control solutions, and gases.
Tangential flow microfiltration is used for medium exchange, perfusion, and harvest.
Downstream applications of microfiltration include
a sterile filtration of buffer products and gases,
which are typically air and nitrogen.
In upstream, virus filtration may be used to protect
cell culture from introduction of viral contaminants in media raw material.
Virus filters are often used in
the downstream processing of cell culture dry product to ensure
removal of both viruses that may enter into
the cell through contaminated raw material or virus particles.
The approximate pore size for virus filters is 20 nanometer to 70 nanometer and
these filters are designed to retain viruses
while allowing protein and buffer components to pass through.
Ultrafiltration membranes typically retain proteins while allowing amino acids,
anti-foam, and buffer components to pass through.
The approximate pore size for ultrafiltration membranes is one nanometer to 20 nanometer
and these membranes are widely used in
downstream to concentrate and buffer exchange the product pool.
It is also used for final formulation of bulk products.
Finally, nanofiltration is used for retaining multi-valent salt,
sugar, amino acids, and antibiotics.
Nanofilters have pore size in the range of 0.1-1 nanometer.