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Printable Handouts
Navigable Slide Index
- Introduction
- Aims and objectives
- What is molecular microbiology?
- Why do we do it?
- How do we do it?
- Cloning – bacterial expression
- Polymerase chain reaction
- Restriction endonucleases
- DNA Ligase
- Cloning
- Transformation and selection
- The lac promoter
- Expression
- Chemostatic bacterial growth
- Insulin
- Hepatitis B vaccine
- Cloning into eukaryotes
- Regulation of GMOs
- Directed reading
Topics Covered
- Molecular biology
- Polymerase chain reaction and the enzymes that make cloning possible
- Examples of applied molecular biology techniques in health and disease
- Cloning in bacteria, yeast and eukaryotes
- GMOs and regulation
Links
Categories:
Therapeutic Areas:
Talk Citation
Cox, J. (2021, August 29). Introduction to applied molecular biology [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved January 26, 2022, from https://hstalks.com/bs/4684/.Publication History
Financial Disclosures
- Dr. Jonathan Cox has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Other Talks in the Therapeutic Area: Infectious Diseases
Transcript
0:00
I'm Dr. Jonathan Cox.
I'm a lecturer in microbiology at Aston University.
This is an introduction to applied molecular biology.
Molecular biology is the subject in which we
manipulate microorganisms in order to be able to get them to do useful things for us.
It's the way in which we can get microorganisms to perform
processes that are useful for us in science and technology.
0:28
The aim of this talk is to introduce the topic of Applied Molecular Biology.
Following this lecture, you should be able to
understand the principles of gene cloning and understand
the principles of recombinant protein production and understand
some examples of commercial and therapeutic recombinant protein production.
0:51
What is molecular microbiology?
It's a branch of microbiology that is devoted to the study of
the molecular basis of the physiological processes that occur in microorganisms.
By understanding these physiological processes,
it enables us to be able to manipulate microorganisms for our own means.
If we understand the molecular pathways,
the molecular process that microorganisms carry out in terms of protein synthesis,
in terms of DNA replication,
in terms of building the physical structures that
they need in order to be able to survive and thrive,
we can effectively hijack that machinery and use
that to produce things that are useful for us in society.
That's what molecular microbiology means.