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Printable Handouts
Navigable Slide Index
- Introduction
- Measurements of cytokine profiles
- Features of cytokines relevant to assays
- Types of assays for cytokines
- Ex vivo assays for cytokines
- Assay selection is a critical decision
- The principle of ELISA
- Cytokine Release Assay (CRA)
- Cytokines antibody arrays with PBMC lysates
- Multiplex Bead Array Assay (MBAA)
- Luminex assay
- Exosomes vs. Plasma
- MBAA summary
- Ultrasensitive cytokine detection
Topics Covered
- The importance of measuring cytokines
- Features of cytokines relevant to assays
- Types of cytokine assays
- Examples of most widely used cytokine assays
- Principles of ELISA -Selecting the correct cytokine assay
- Multiplex analyses and cytokine profiles
Talk Citation
Whiteside, T. (2022, January 31). Assays for cytokines and cytokine assay performance 1 [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 10, 2024, from https://doi.org/10.69645/GYVW2334.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Theresa Whiteside has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Assays for cytokines and cytokine assay performance 1
Published on January 31, 2022
41 min
A selection of talks on Immunology & Inflammation
Transcript
Please wait while the transcript is being prepared...
0:00
Hi, I am Theresa Whiteside.
I'm a professor of pathology at
the University of Pittsburgh and I work at the Hillman Cancer Center.
I will be talking to you today about assays for cytokines
and the performance of this assay that gives us rational, good data.
0:26
Cytokines and chemokines play a critical role in the pathogenesis of human diseases.
Cytokines and chemokines are similar in many characteristics,
such as their small size,
which varies between 5 and 20 kDa,
and they are slightly different in structure,
but they perform different functions.
While cytokines mediate immune responses and are involved in inflammation,
chemokines regulate the migration of cells and their chemotaxis,
their accumulations in various tissues.
The levels of cytokines and chemokines change under
pathologic conditions and the availability of
biologically relevant and reliable methods for their assessment in
human body fluids and tissues is critically important and clinically important.
There are many different known cytokines.
I think we know about 50 of them,
and there is clearly a division of labor among the cytokines and
that requires methods for defining cytokine profiles and cytokine networks.
Cytokines tend to work like networks,
meaning that one cytokine induces the appearance of other cytokines.
Perhaps the best way to illustrate this is to talk
a little bit about a CD4 positive T cell development.
CD4 positive helper T cells have many different subsets,
so they are Th1 and Th2 cells,
Th9, and Th17 regulatory helper T cells,
as well as follicular helper T cells.
Each set of these CD4 positive T cells,
is characterized by a different set of cytokines they produce,
and by the presence of master transcription factors that define this subset.
The transcription factors are listed underneath each of these sets here and
the cytokines or set of cytokines each set produces are listed on the right-hand side.
For example, you can see that the Th1 set which mediates,
which is pro-inflammatory subsets produces
interferon gamma IL-12 while Th2 subsets produce Ion2,
Ion4, and lon13, et cetera.
This is important because these sets or networks of cytokines,
these subsets determine their role and regulate their functions.