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Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- Animal models through the ages
- How to choose animal models?
- Advantage of rodents
- Mice models (1)
- Mice models (2)
- Induced models
- Genetically engineered models
- Genetically engineered rodents in research
- Genetic engineering, techniques
- Spontaneous mutations
- Chemical mutagenesis
- Insertional transgenesis: pronuclear injection
- Insertional transgenesis: pronuclear injection movie
- Insertional transgenesis: pros
- Insertional transgenesis: cons
- Targeted transgenesis: homologous recombination
- Targeted transgenesis: ES cells
- Targeted transgenesis: principle
- Targeted transgenesis: injection (movie)
- Targeted transgenesis: pros
- Targeted transgenesis: cons
- Genetic engineering, strategies
- Insertional transgenesis: strategies
- Targeted transgenesis: knock out
- Targeted transgenesis: knock in
- Targeted transgenesis: conditional strategies (1)
- Targeted transgenesis: conditional strategies (2)
- Targeted transgenesis: inducible strategies (1)
- Targeted transgenesis: inducible strategies (2)
- TetOff system
- TetOn system
- Inducible strategies: pros/cons
- RNA interference: principle
- RNA interference: pros/cons
- Genetic switch
- More strategies
- Latest developments in genetic engineering
- Targeted endonucleases (1)
- Possible genome editing outcomes
- Possibilities that arise from different editing types
- Targeted endonucleases (2)
- The CRISPR-CAS9 system (1)
- The CRISPR-CAS9 system (2)
- Line creation with CRISPR-CAS9
- Direct production of double homozygous animals
- Technological breakthrough
- Applications in mice and rats
- Genomic studies (1)
- Genomic studies (2)
- Generating pathological models
- Drug validation
- Humanization (1)
- Humanization (2)
- A model for every single need
- International KO mouse consortium
- International mouse strain resource
- Zebrafish (Danio Rerio)
- Targeted endonuclease for other species
- Applications in other species
- Bioproduction
- Conclusion
- Thank you
Topics Covered
- Choosing the right animal models
- Inserting a transgene into an organism model (random or targeted integration)
- Controlling space and time of transgene expression
- Genome editing via CRISPR-Cas9
- Examples of genetically engineered models
- Latest developments in genetic engineering
Links
Series:
Categories:
Talk Citation
Gomas, E. (2015, February 11). Creating animal models by genetic techniques [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 13, 2024, from https://doi.org/10.69645/ISIS4155.Export Citation (RIS)
Publication History
Financial Disclosures
- Mr. Emmanuel Gomas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Other Talks in the Series: Animal Models in Biomedical Research
Transcript
Please wait while the transcript is being prepared...
0:00
Good morning, everybody.
Thank you very much for attending
this talk about "Creating Animal
Models by Genetic Techniques".
My name is Emmanuel Gomas and
I've worked in the last 16 years
now in the field of
transgenic animals,
in particular, transgenic mice
and rats, both in academic labs,
but also in a private company.
In the last year I became
a consultant trying to help
and providing help to
laboratories and facilities
to improve their colony
management and facility management.
0:32
In the seminar series about
animal models embedded
in medical research,
it's important to have
a talk about transgenic animals,
because these transgenic animals
now are a big part of animal
models and may be one of the most
important part in animal models.
So it's very important to
understand where they come from,
why they are there, inside of
the evolution of animal models
through the ages.
But also it's important to know
how, technically, these animals are
engineered and the type
of genetic strategies
we can use in genetic engineering.
Also I will spend some
time on the latest
developments in genetic
engineering, occurring
in the last two or three years.
And I will finish
by speaking about some
applications of transgenic animals,
some state of the
art, and some points
in the future as a conclusion.
1:21
It's important to replace transgenic animal
models in the evolution of animal
models needs through the ages.
Because if we start in the early
steps of animal model experiments--
let's say with Claude Bernard or in
the Pasteur Institute as an early beginning
through today, there is a wide
range of use of animal models
and a huge evolution of
the use of animal models.