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Printable Handouts
Navigable Slide Index
- Introduction
- Why test for autoantibodies?
- Earliest methods for autoantibody testing
- More recent methods for autoantibody testing
- Antinuclear antibody test (ANA)
- Immunofluorescence assay (IFA)
- Performing an IFA (part 1)
- Performing an IFA (part 2)
- Performing an IFA (part 3)
- Multiple uses for IFA testing
- Cell biology
- Mitosis
- Negative ANA
- Homogeneous pattern
- Speckled pattern (anti-SS-A)
- Cytoplasmic staining (anti-Jo-1)
- Nucleolar pattern
- Centromere pattern
- Crithidia luciliae
- Negative anti-DNA (Crithidia luciliae)
- Positive anti-DNA (Crithidia luciliae)
- Organ-specific IFA (example)
- Anti-LKM 1 antibodies
- Enzyme immunoassay
- Performing an ELISA (part 1)
- Performing an ELISA (part 2)
- Performing an ELISA (part 3)
- Performing an ELISA (part 4)
- Close-up view of an EIA strip
- Major antigens in systemic autoimmune diseases
- Methods for detection of specific autoantibodies
- Immunoprecipitation for nucleic acids
- Limitations of IFA methods
- Limitations of EIA methods
- Current trends in autoantibody testing
- Array technology for multiplex testing (1)
- Bead arrays
- Determinations of antibodies in a SLE patient
- Array technology for multiplex testing (2)
- Line assays and planar arrays
- Autoantibodies detected with a line immunoassay
- Detection of IgG/IgM antibodies on a planar array
- Diagnostic tests used to help identify diseases
- Conclusions
Topics Covered
- Methods for autoimmunity testing
- Significant antigens in systemic autoimmune disease
- Arrays and other new technologies
- Testing for additional autoimmune diseases
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Binder, S. (2018, July 31). Diagnostic methods in autoimmunity [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 5, 2024, from https://doi.org/10.69645/VYQA6115.Export Citation (RIS)
Publication History
Financial Disclosures
- Mr. Steven Binder has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Immunology
Transcript
Please wait while the transcript is being prepared...
0:00
Hello, my name is Steven Binder,
and I'm the Senior Director of technology development at Bio-Rad Laboratories.
Today we're going to be discussing diagnostic methods used to measure autoantibodies,
which are important for autoimmune disease.
There are many methods available for autoimmune testing,
and it's important to understand that these methods are not highly standardized.
Today you can get results for glucose or cholesterol from
almost any clinical laboratory with excellent agreement
between laboratories in very good precision and reproducibility.
This is not yet the case for autoimmune testing.
Of course, the number of methods available are a major source of this variation,
but the reason we have this state today is, there are
both very large labs and very small labs doing the testing.
Further there is a lot of biological variation,
since a human autoantibody is not a well-defined molecule,
like cholesterol, and can vary from individual to individual.
So given these complexities,
I'm going to try and share some information with you today about the sorts of
tests that are available for autoimmune testing in use today.
1:20
Because many autoimmune diseases are difficult to diagnose,
a test with high sensitivity would be useful to rule out these diseases.
There are however only a few diseases where autoantibodies are observed
in even 90 percent of cases.
So the laboratory results alone, are rarely sufficient to rule out disease.
A more common situation is the use of an autoimmune test to support a diagnosis,
such as the detection of
anti-SSA and anti-SSB antibodies, when Sjogren's syndrome is suspected.
Another common use for these tests is assistance in differential diagnosis.
For example, the detection of different anchor patterns can assist
the rheumatologist in the assessment of a patient with possible vasculitis.
Finally using serial blood collections to follow
increases and decreases in antibody levels,
for example, the anti-dsDNA levels in SLE patients, can be
a useful approach for monitoring
disease activity and determining the efficacy of treatment.