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1. Introduction
2. "True" fungal pathogens
3. Reproduction, ecology and infection
4. Sex, ecology and infection in fungi
5. Sex in fungal pathogens
6. Fungi reproduce in a variety of ways
7. Importance of sex and recombination
8. How can we prove sex in fungal pathogens
9. Assessing sexual vs. clonal reproduction (1)
10. Assessing sexual vs. clonal reproduction (2)
11. Complications: sexual populations can look clonal
12. Complications: clonal populations can look sexual
13. Step 1: develop multilocus genotype data set
14. Step 2: phylogenetic analysis
15. Step 3: look for linkage between loci
16. Step 4: compare datasets
17. Does sex happen in fungal pathogens?
18. Histoplasma capsulatum (1)
19. Histoplasma capsulatum (2)
20. Coccidioides immitis (1)
21. Coccidioides immitis (2)
22. Pathogenic Cryptococcus
23. Pathogenic species of Cryptococcus
24. C. gattii molecular genotypes
25. Molecular genotypes VGI and VGII
26. Australian disease statistics
27. VGII and the Vancouver Is. outbreak
28. The Cryptococcus lifecycle
29. The mating type locus
30. Cryptococcus ecology and infection
31. How Cryptococcus spores are formed?
32. Questions and aims of study
33. Analysis 1: Australian environmental populations
34. Mating type bias in C. gattii
35. Cluster analysis from 34 AFLP fragments
36. Analysis of cluster 3 only
37. PCA associates genotypes with trees
38. The environmental Renmark population
39. The Eucalyptus: a true host of Cryptococcus?
40. Clinical vs. environmental populations of C. gatii
41. Analysis 2: clinical populations - Australia/PNG
42. Molecular type and mating type
43. Phylogenetic and recombination analysis (IA)
44. Recombination aligns with fertility
45. Genetic differentiation among phylogroups
46. MLSTs: two distinct VGII groups
47. VG groups differ in fertility
48. A quandry...
49. VGI - why no sex?
50. VGII - how sex?
51. Sex and recombination between alpha cells only
52. alpha-alpha mating and the Vancouver Is outbreak
53. Refining population choice and recombination?
54. Environmental populations revisited...
55. Recombination in hollows with both mating types
56. Recombination in hollow with a mating type only
57. Pairings of Renmark isolates
58. Does recombination happen on a global scale?
59. Recombination on a global scale: MLST loci
60. MLST data from Fraser et al., 2005
61. Pairwise compatibility test for global VGI isolates
62. Pairwise compatibility test for global VGII isolates
63. C. gattii sex, ecology and infection: conclusions
64. Finding sex and recombination - the final word
65. Acknowledgements

Topics Covered

• The true fungal pathogens: reproduction at the interface of ecology and infection
• Sex in fungal pathogens: why do we want to know and how can we prove it?
• Molecular methods of assessing sexual versus clonal reproduction
• How does sex in fungal pathogens happen?
• Recombination in a sexual fungus: Histoplasma capsulatum
• Recombination in an "asexual" fungus: Coccidioides immitis
• Clonality and recombination in a sexual fungus: Cryptococcus

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Categories:

• Agriculture & Environmental Sciences
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Talk Information

• Published on March 31, 2010
• Updated on July 22, 2010