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Printable Handouts
Navigable Slide Index
- Introduction
- Designing small animal imaging facilities
- What do you plan to image?
- Imaging methods
- What species to use
- Regulatory oversight
- Infectious and carcinogenic agents
- Immunocompromised imaging (1)
- What level of service?
- Who will do the work?
- UCLA imaging experiments: two roles
- Animal handling training
- Systems and personnel requirements
- Planning the flow of the workspace
- New Crump Imaging Center
- PET-CT space
- Vivarium
- Support equipment
- Computer network configuration
- Database and image management
- Web-based scheduling and image retrieval
- Archiving and data retrieval
- Operational considerations
- Preclinical molecular imaging
- Why use animals?
- What is being imaged?
- Experimental design issues
- Glucose effects on FDG (1)
- Glucose effects on FDG (2)
- Probe uptake: anesthesia effects
- Isoflurane effects on FDG uptake
- Conventional anesthesia configuration
- Anesthesia support
- Anesthesia induction boxes
- Immunocompromised imaging (2)
- Staging mice
- Temperature control
- Brown fat uptake
- Temperature effects in tumor bearing mice
- Heating options
- Intraperitoneal injections
- Tail vein injections
- Dynamic imaging prep
- Blood sampling options
- Tail blood sampling: tail 'poke'
- Example images
- Biodistribution and radiation dosimetry
- Oncology imaging
- D2 receptor ligand imaging using microPET
- Perfusion and metabolism microPET images
- microPET: oncology - PET-only
- microPET: oncology - dual modality imaging
- Dynamic imaging of FHBG
- Fast temporal imaging: first past transit
- Imaging cancer using PET-reporter gene & FHBG
- Functional and morphological imaging
- Adenovirus validation in lymph node metastasis
- Optical imaging: bioluminescence/fluorescence
- Fluorescent growth factor on implanted scaffold
- Bone formation assessed using microCT
- Getting it right
- References and acknowledgements
Topics Covered
- Designing small animal imaging facilities
- Imaging methods
- Regulatory oversight
- Infectious and carcinogenic agents
- UCLA imaging experiments
- Animal handling training
- Systems and personnel requirements
- Planning the flow of the workspace
- Database and image management
- Archiving and data retrieval
- Operational considerations
- Preclinical molecular imaging: why use animals?
- Experimental design issues
- Anaesthesia
- Heating options
- Blood sampling
- Case studies
- Optical imaging
- Getting it right
Talk Citation
Stout, D. (2010, April 1). Pre-clinical imaging centers: design, animal handling and examples [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved January 28, 2021, from https://hstalks.com/bs/1672/.Publication History
Financial Disclosures
- Dr. David Stout has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Pre-clinical imaging centers: design, animal handling and examples
Published on April 1, 2010
49 min