Registration for a live webinar on 'The role of T cells in COVID from asymptomatic to severe outcomes' is now open.
See webinar detailsWe noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
- RNAi-Pathway Components and Mechanisms
-
1. Activation of gene expression by double-stranded RNAs
- Prof. Long-Cheng Li
-
2. MicroRNA biogenesis
- Dr. Narry Kim
-
3. Dicer and R2D2
- Dr. Young Sik Lee
-
4. Mammalian microRNA assembly and function
- Prof. Zissimos Mourelatos
-
5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer
- Prof. Leemor Joshua-Tor
- Roles for RNAi in Development and Anti-Viral Defense
-
6. Functional roles of RNAi in C. elegans
- Dr. Eric Miska
-
7. RNAi and heterochromatin in plants and fission yeast
- Prof. Robert Martienssen
-
8. RNA interference functions and mechanisms in animals
- Prof. Richard Carthew
-
9. Specific functional roles in mammals
- Dr. Michael McManus
- RNAi Design Rules for Applications in Humans
-
10. siRNA on and off-target
- Prof. Anastasia Khvorova
-
11. Cell signalling pathways and RNAi
- Mr. Michael O'Grady
-
12. RNAi: key technological aspects
- Dr. Dmitry Samarsky
- Dr. Ciaran Faherty
-
13. RNAi specificity: how big of an issue is it?
- Dr. Peter Welch
- Applications of RNAi in the Treatment of Human Diseases
-
14. siRNA therapeutics
- Prof. John Rossi
- Mr. Daniel J. Rossi
-
15. RNAi for neurological diseases
- Prof. Beverly L. Davidson
-
16. RNA interference and cancer: a revolution?
- Dr. Annick Harel-Bellan
- Archived Lectures *These may not cover the latest advances in the field
-
17. RNAi and viral heart infections
- Dr. Jens Kurreck
-
18. RNA interference for neurodegenerative diseases
- Prof. Beverly L. Davidson
-
19. Activation of gene expression by double-stranded RNA
- Prof. David Corey
-
20. New designs for siRNAs as Dicer substrate
- Dr. Mark Behlke
-
21. RNAi in the model system Drosophila
- Prof. Richard Carthew
-
23. Therapeutic delivery of RNAi
- Dr. Patrick Lu
-
24. Mechanistic aspects and therapeutic applications of RNAi
- Prof. John Rossi
Printable Handouts
Navigable Slide Index
- Introduction
- Related publications
- Outline
- RNA interference
- Dogma
- Can duplex RNA also recognize DNA?
- agRNAs target the PRB transcription start site
- agRNAs are duplex RNAs
- agRNAs inhibit protein expression
- Inhibiting gene expression by using agRNAs
- The Argonaute protein (Ago)
- Inhibiting Ago2 blocks agRNAs silencing
- Synthetic RNAs can control promoter DNA
- Gene activation by promoter-targeted RNAs
- Structure activity analysis: unexpected results
- Hypothesis: 'inactive' RNA can activate genes
- Testing the hypothesis in MCF7 cells
- An RNA activates PR expression in MCF7 cells
- What is the activation mechanism?
- Inactive RNAs are competitive inhibitors
- Clinical development of duplex RNA
- Conclusions: RNA-mediated gene activation
- Noncoding RNAs as molecular targets
- Two possible mechanisms for activation
- Most of the human genome is transcribed
- What is a gene? two views
- Antisense transcript overlaps PR promoter
- The RIP technique
- agRNAs recruit AGO to an antisense transcript
- Conclusions from published work
- RNA complementary to sequences beyond 3'-UTR
- The 3' end: two views
- PR mRNA was insufficiently characterized
- A PR transcript ending around 13037
- Quantitative RT-PCR confirmation of the 3' end
- 3'-RACE defined the 3' end of PR mRNA
- 3' noncoding sense transcript
- PR is silenced in T47D cells by 3' agRNA
- 3' agRNA reduces PR mRNA and pre-mRNA levels
- 3' agRNA effect on 3' and 5' noncoding transcripts
- 3' silencing agRNA reduces Pol2 recruitment
- Noncoding transcript has target sites for 3' agRNA
- Inhibitory 3' agRNA recruits AGO2 protein
- PR is activated in MCF7 cells by a 3' agRNA
- 3' activating agRNA increases mRNA levels
- 3' activating agRNAs increase Pol2 recruitment
- Activating 3' agRNA recruits AGO protein
- miRNAs complementary to gene promoters
- Expanding the pool of candidate miRNAs
- miRNAs complementary to the PR gene promoter
- miRNA mimics inhibit PR protein expression
- Inhibition of PR protein by miR-423-5p mimic
- miR-423-5p mimic inhibits PR mRNA expression
- Complementarity to regions downstream of PR
- A step towards endogenous 3' agRNAs?
- Inhibition by miR-193b mimic is dose dependent
- Inhibition of PR mRNA and pre-mRNA expression
- 3' agRNAs conclusions
- agRNAs target seq. 100,000 bases distant
- The DNA looping theory
- Are 5' and 3' of the PR gene in close proximity?
- Experiments with PR13580
- Experiments with PR9
- Experiments with PR13515
- Experiments with PR11
- 5' and 3' ncRNA are in a complex
- Proposed model
- Acknowledgements
Topics Covered
- RNA-mediated recognition of chromosomal DNA
- Gene silencing
- Gene activation
- Mechanism of recognition of promoter DNA sequences by duplex RNAs
- Recognition of sequences beyond the 3'-termini of genes by duplex RNAs
Talk Citation
Corey, D. (2010, February 9). Activation of gene expression by double-stranded RNA [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 27, 2024, from https://hstalks.com/bs/1624/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. David Corey has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.