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1. Introduction
2. T cell activation
3. Dendritic cells (DC)
4. Immune activation (1)
5. Pattern recognition
6. Bug recognition by Toll-like receptors in mammals
7. PRRs: coupling innate and adaptive immunity
8. Immune activation (2)
9. Viruses induce type I/III interferons
10. Induction of type I interferons is pattern recognition
11. Pattern recognition of viruses
12. IFN-alpha production and cytoplasmic dsRNA
13. IFN-alpha/beta induction by cytoplasmic dsRNA
14. Influenza virus
15. Influenza NS1 protein blocks IFN-a/b production
16. Cytoplasmic recognition of flu
17. Induction of IFN-a does not correlate with dsRNA
18. RIG-I mediated flu sensing
19. Transfection with flu vRNA is sufficient to elicit IFN
20. RIG-I binds directly to flu vRNA
21. RIG-I mediated sensing of flu genomes
22. Induction of type I/III interferons
23. Recognition is specific to vRNA
24. CIP abrogates the ability of flu vRNA to induce IFN
25. Conclusions (1)
26. 5' PPP RNA binds to the CTD of RIG-I
27. Why RIG-I doesn't mediate responses to EMCV
28. What about self ssRNA?
29. The actual RIG-I agonist during virus infection
30. IFN-a/b induction by viruses - Cytosolic pathways
31. Mouse DC subsets
32. pDC make lots of IFN-a in response to wild type flu
33. Conclusions (2)
34. pDC responses to flu require TLR7
35. Endosomal recognition of viruses
36. Virus recognition pathways
37. TLR7 recognises poly U clusters
38. TLR7 recognises self mRNA
39. Conclusions (3)
40. What about apoptotic cells? (1)
41. Spleen DC subsets
42. Scavenging by CD8-alpha positive DC
43. What about apoptotic cells? (2)
44. CD8a+ DC do not express TLR7 but express TLR3
45. DC "decode" signs of infection within dying cells
46. Detecting virally-infected cells
47. Replication of some viruses generates dsRNA
48. DC activation by virally-infected cells
49. Virus-infected cells induce CTL responses
50. CTL priming after TLR3-mediated dsRNA sensing
51. Summary
52. Acknowledgements

Topics Covered

• Induction of the adaptive Immunity
• Pattern recognition receptors: coupling innate to adaptive immunity
• Induction of type I IFNs: an example of recognition of viral patterns
• Cytosolic pathway for influenza virus recognition and inhibition by the viral
• NSI protein
• RIG-I-mediated recognition of influenza virus genomes and 5'-PPP-RNA
• Cytosolic 5'-PPP-RNA as a PAMP
• Plasmacytoid DC and the discovery of a second pathway for innate recognition of influenza virus
• Endosomal pathways for virus recognition: TLR7, TLR9
• TLR3 as a sensor of virally-infected cells

Links

Series:

• Innate Immunity

Categories:

• Immunology
• Microbiology

Therapeutic Areas:

• Immunology & Inflammation
• Infectious Diseases

Talk Citation

Publication History

• Published on June 30, 2009

Financial Disclosures

• Prof. Caetano Reis e Sousa has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.