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Printable Handouts
Navigable Slide Index
- Introduction
- The first attempt of intravital microscopy
- Intravital microscopy
- White cells in cremasteric venule
- The leukocyte recruitment cascade (1)
- Proposed mechanism for induced rolling
- Role of P-selectin in leukocyte rolling
- Selectins
- Beta2 integrins (CD18)
- LFA-1 and Mac-1 function (1)
- LFA-1 and Mac-1 function (2)
- Leukocyte recruitment cascade (2)
- Adhesion of neutrophils is dependent on LFA-1
- In vivo time lapse: leukocyte crawling
- In vivo time lapse in Mac-1 deficient cells
- Crawling is dependent on Mac-1
- In vivo PECAM-labeled junctions
- In vivo confocal microscopy
- WT neutrophils transmigrate at junctions
- Mac-1 deficiency effect on migration
- Junctional migration
- Mac-1 deficiency and neutrophils emigration time
- Leukocyte recruitment cascade (3)
- The liver vascular bed
- Liver vascular bed - control
- Vascular bed - an inflamed liver
- Neutrophils and adhesion molecules in the liver
- A role for adhesion in sinusoids?
- Endothelial adhesion molecule expression in vivo
- Adhesion molecule expression in various organs
- Hyaluronan expression in the liver
- CD44 is a receptor for HA
- CD44 and HA mediate neutrophil adhesion
- CD44 is not important in hepatic venules
- Occlusion of sinusoids
- CD44 and sinusoidal perfusion
- Sinusoidal leukocyte adhesion is reversible
- Reverse adhesion increases sinusoidal perfusion
- Generation of Th1 and TH2 cells
- Con A-induced liver inflammation
- Multiple compartments for leukocyte recruitment
- Vascular adhesion protein (VAP)-1 (1)
- Vascular adhesion protein (VAP)-1 (2)
- Hepatic VAP-1 expression
- Th1 and Th2 lymphocyte adhesion
- Alpha4 integrin mediates rolling and adhesion
- Complete healing ileum
- Clinical response to natalizumab
- Brain preparation
- Control brain
- Inflamed brain
- T cell-subset recruitment to the CNS in EAE
- Mechanisms of Th1 T cell rolling
- Mechanisms of Th2 T cell rolling
- Th1 and Th2 cells bind P-selectin
- Th1 and Th2 cells can bind selectins
- Monitoring of blood vessels by monocytes
- NKT cells in vascular bed of liver from CXCR6 mice
- NKT cells from CXCR6 mice - enlarged video
- The effect of systemic LPS on cell rolling
- Rapid lung infiltration by neutrophils
- Intravital microscopy of the lung
- Leukocyte trafficking within the lung
- The effect of systemic LPS on the lung
- Neutrophils get stuck in the lung capillary
- Which molecules mediate neutrophil recruitment?
- How do neutrophils enter lungs?
- Neutrophils are activated in endotoxemic blood
- Scheme of the effect of LPS
- Both leukocytes and endothelium respond to LPS
- Bone marrow transfer protocol
- Bone marrow transfer experiment
- LPS effect on TLR4 leukocytes
- Systemic LPS and leukocyte activation
- Candidates for neutrophil recruitment
- Acknowledgements
Topics Covered
- Leukocyte recruitmen
- A multi-step recruitment that involves rolling adhesion, crawling to junctions and emigration
- Role of selectins and integrin subunits in this process
- Role for other adhesion molecules in liver, lung and potentially other organs
- Constitutive patrolling of immune cells in various tissues
- Role for sentinel cells such as endothelium, mast cells and macrophage in inducing recruitment into tissues
Talk Citation
Kubes, P. (2009, June 30). Leukocyte recruitment in vivo [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 2, 2024, from https://doi.org/10.69645/OQQL3026.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Paul Kubes has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.