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1. Introduction
2. The first attempt of intravital microscopy
3. Intravital microscopy
4. White cells in cremasteric venule
5. The leukocyte recruitment cascade (1)
6. Proposed mechanism for induced rolling
7. Role of P-selectin in leukocyte rolling
8. Selectins
9. Beta2 integrins (CD18)
10. LFA-1 and Mac-1 function (1)
11. LFA-1 and Mac-1 function (2)
12. Leukocyte recruitment cascade (2)
13. Adhesion of neutrophils is dependent on LFA-1
14. In vivo time lapse: leukocyte crawling
15. In vivo time lapse in Mac-1 deficient cells
16. Crawling is dependent on Mac-1
17. In vivo PECAM-labeled junctions
18. In vivo confocal microscopy
19. WT neutrophils transmigrate at junctions
20. Mac-1 deficiency effect on migration
21. Junctional migration
22. Mac-1 deficiency and neutrophils emigration time
23. Leukocyte recruitment cascade (3)
24. The liver vascular bed
25. Liver vascular bed - control
26. Vascular bed - an inflamed liver
27. Neutrophils and adhesion molecules in the liver
28. A role for adhesion in sinusoids?
29. Endothelial adhesion molecule expression in vivo
30. Adhesion molecule expression in various organs
31. Hyaluronan expression in the liver
32. CD44 is a receptor for HA
33. CD44 and HA mediate neutrophil adhesion
34. CD44 is not important in hepatic venules
35. Occlusion of sinusoids
36. CD44 and sinusoidal perfusion
37. Sinusoidal leukocyte adhesion is reversible
38. Reverse adhesion increases sinusoidal perfusion
39. Generation of Th1 and TH2 cells
40. Con A-induced liver inflammation
41. Multiple compartments for leukocyte recruitment
42. Vascular adhesion protein (VAP)-1 (1)
43. Vascular adhesion protein (VAP)-1 (2)
44. Hepatic VAP-1 expression
45. Th1 and Th2 lymphocyte adhesion
46. Alpha4 integrin mediates rolling and adhesion
47. Complete healing ileum
48. Clinical response to natalizumab
49. Brain preparation
50. Control brain
51. Inflamed brain
52. T cell-subset recruitment to the CNS in EAE
53. Mechanisms of Th1 T cell rolling
54. Mechanisms of Th2 T cell rolling
55. Th1 and Th2 cells bind P-selectin
56. Th1 and Th2 cells can bind selectins
57. Monitoring of blood vessels by monocytes
58. NKT cells in vascular bed of liver from CXCR6 mice
59. NKT cells from CXCR6 mice - enlarged video
60. The effect of systemic LPS on cell rolling
61. Rapid lung infiltration by neutrophils
62. Intravital microscopy of the lung
63. Leukocyte trafficking within the lung
64. The effect of systemic LPS on the lung
65. Neutrophils get stuck in the lung capillary
66. Which molecules mediate neutrophil recruitment?
67. How do neutrophils enter lungs?
68. Neutrophils are activated in endotoxemic blood
69. Scheme of the effect of LPS
70. Both leukocytes and endothelium respond to LPS
71. Bone marrow transfer protocol
72. Bone marrow transfer experiment
73. LPS effect on TLR4 leukocytes
74. Systemic LPS and leukocyte activation
75. Candidates for neutrophil recruitment
76. Acknowledgements

Topics Covered

• Leukocyte recruitmen
• A multi-step recruitment that involves rolling adhesion, crawling to junctions and emigration
• Role of selectins and integrin subunits in this process
• Role for other adhesion molecules in liver, lung and potentially other organs
• Constitutive patrolling of immune cells in various tissues
• Role for sentinel cells such as endothelium, mast cells and macrophage in inducing recruitment into tissues

Links

Series:

• Innate Immunity

Categories:

• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on June 30, 2009

Financial Disclosures

• Prof. Paul Kubes has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.