Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Mononuclear phagocyte system
3. Macrophages.com website
4. Image from macrophages.com
5. Localization of macrophages in tissues
6. Marker for the mononuclear phagocyte system
7. EGFP expression in all MacGreen macrophages
8. The MacGreen mouse: possible applications
9. Intravital imaging of MacGreen mouse hind joint
10. Macrophages and bone
11. Using MacGreen to study macrophages in bone
12. Distribution of BAMs on bone surface
13. Macrophages in blood vessels of MacGreen
14. Binary expression system using CSF-1R promoter
15. The binary system is functional in macrophages
16. Using MacBlue to study tissue remodeling
17. ECFP expression in microglia of MacBlue mice
18. Expression of a tagged Schlafen4 in primary cells
19. Cellular systems biology
20. The FANTOM consortium
21. CAGE technology
22. TAG clusters identify TSS and promoters
23. CAGE is a form of expression profiling
24. Macrophage-specific promoters and their biology
25. Biology of gelsolin
26. Two major promoters in the gelsolin locus
27. The two promoters lead to two forms of gelsolin
28. Gelsolin promoter 2: lung-enriched
29. Conservation of promoter 2 in mouse and human
30. Promoter 1: macrophage-enriched, broad type
31. Conservation of promoter 1 in mouse and human
32. What do we learn from gelsolin?
33. Gelsolin-related protein (CapG) is also complex
34. Using promoter analysis to study IGF-1
35. Expression patterns: macrophage vs. osteoblast
36. The complicated IGF-1 promoter region
37. IGF-1 promoter conservation across species
38. CSF-1 deficient animal is also IGF-1 deficient
39. THP-1: a model for cellular differentiation
40. THP-1: PMA differentiation time-course
41. PMA induces macrophage markers
42. PU1- one gene, three promoters
43. Integrating data from THP-1 differentiation analysis
44. Motif activity analysis
45. Example of a network of transcription factors
46. So, what does it all mean?
47. Credits

Topics Covered

• Marker for the mononuclear phagocyte system
• CSF-1R
• EGFP
• The MacGreen mouse
• Macrophages and bone
• MacBlue mice
• Association of macrophages with tissue remodelling during embryonic development
• Cellular systems biology
• Transcriptional landscape of the mammalian genome
• CAGE technology
• Expression profiling
• Macrophage-specific promoters and their associated biology
• Biology of Gelsolin
• Gelsolin-related protein
• IGF-1 promoter region
• THP-1: a model for cellular differentiation
• PMA induces macrophage markers
• Motif activity analysis

Links

Series:

• Innate Immunity

Categories:

• Immunology

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on June 30, 2009
• Reviewed on July 23, 2020

Financial Disclosures

• Prof. David Hume has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.