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- Co-ordination of G1 Progression
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1. START control in yeast
- Prof. Curt Wittenberg
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2. The pRB/E2F pathway
- Prof. Jacqueline Lees
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3. Cell cycle control by the ubiquitin system in mammals
- Prof. Michele Pagano
- Chromosome Duplication
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4. Replication licensing
- Prof. Julian Blow
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5. Initiation of DNA replication
- Prof. Bruce Stillman
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6. Regulation of replication fork progression and stability
- Dr. Luis Aragón
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7. Nucleosome assembly during DNA replication
- Dr. Alain Verreault
- Preparing for Mitosis
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8. Sister chromatid cohesion: simple concept, complex reality
- Prof. Douglas Koshland
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9. Mitotic chromosome condensation
- Prof. Andrew Belmont
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10. Centrosome duplication and separation in animal cells
- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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11. Bipolar spindle assembly
- Dr. Eric Karsenti
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12. Chromosome bi-orientation in yeast
- Prof. Mike Stark
- Prof. Tomo Tanaka
- Mitotic Exit and Cytokinesis
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14. Cleavage furrow formation and ingression during animal cytokinesis
- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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15. The DNA damage response
- Dr. Vincenzo Costanzo
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16. The spindle checkpoint
- Dr. Kevin Hardwick
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17. Spindle movement and checkpoint control during mitosis in yeast
- Prof. John Cooper
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18. The G2/M transition
- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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19. Mouse models to investigate cell cycle and cancer
- Dr. Philipp Kaldis
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20. Cell cycle: a complex network of signals regulating cell proliferation
- Prof. Antonio Giordano
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21. Drug discovery and target validation in the p53 pathway
- Prof. Sir David Lane
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22. Role and regulation of Cdk inhibitors in development and cancer
- Prof. Martine Roussel
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24. The Myc transcription factor network
- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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25. Recombination and the formation of chiasmata in meiosis
- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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26. Geometric regulation of kinetochore orientation
- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- Cell cycle checkpoints for partitioning the genome
- Budding yeast as a model system
- Nuclei segregation in yeast (1)
- Nuclei segregation in yeast (2)
- Spindle position checkpoint
- Evidence for a spindle position checkpoint (1)
- Evidence for a spindle position checkpoint (2)
- Before spindle moves into neck
- After spindle moves into neck
- Movie assay for checkpoint - normal
- Movie assay for checkpoint - delayed
- Movie assay for checkpoint - inappropriate
- Movie assay for mitotic cell
- GAP mutants show a complete loss of checkpoint
- Lte1p - Putative GEF for Tem1
- Inappropriate mitotic exit (1)
- Inappropriate mitotic exit (2)
- Putative MT sensor of neck
- Screen for checkpoint mutations
- Septins (1)
- Septins (2)
- Dual role of septins (1)
- Dual role of septins (2)
- Flow of information controlling mitotic exit (1)
- Lte1 interacting genes and proteins
- Bud6 mediates MT capture
- Potential biochemical pathway
- Null mutants have checkpoint defects
- Flow of information controlling mitotic exit (2)
- Defects are suppressed by loss of Lte1
- Double mutants aren't worse than single mutants
- Bud6 interaction with MT ends
- Mutations that affect Bud6/MT interactions
- Kip2 null mutant has a checkpoint phenotype
- Overexpression analysis for pathway order
- Mitotic exit with a MT present in the neck
- Conclusions for Bud6
- Inhibiting Lte1 is important in the cold
- Cdc5 and Kin4 control mitotic exit
- Future questions and directions
Topics Covered
- Cell cycle checkpoints for partitioning the genome
- Budding yeast as a model system for the study of genome distribution
- How nuclei are segregated in yeast
- Evidence for a spindle position checkpoint
- Model for checkpoint based on Tem1
- Movie assay for checkpoint
- Search for upstream inputs
- Screen for checkpoint mutants
- Septins dual role in checkpoint
- Inputs to Lte1
- Bud6 and microtubule capture
- Potential pathway for Bud6
- Bud6 and microtubule-binding proteins
- Overexpression analysis
- Inhibition of Lte1 in the cold
Talk Citation
Cooper, J. (2009, April 30). Spindle movement and checkpoint control during mitosis in yeast [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2024, from https://hstalks.com/bs/1267/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. John Cooper has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.