• Overview of Chaperone Networks
• 41 min
  1. Mapping the molecular chaperone interaction network in yeast

  • Prof. Walid A. Houry
• 30 min
  2. The interaction network of the GroEL chaperonin

  • Prof. Dr. F. Ulrich Hartl
• 61 min
  3. Human heat shock protein families

  • Prof. Herman Kampinga
• 42 min
  4. Extracellular proteostasis: an emerging field

  • Prof. Mark Wilson
• 36 min
  5. Protein homeostasis and the chaperone interaction network in Arabidopsis thaliana

  • Prof. Rongmin Zhao
• Proteasome Networks
• 64 min
  6. Biogenesis of the eukaryotic proteasome

  • Prof. Mark Hochstrasser
• Understanding Intrinsically Disordered Proteins in Protein Homeostasis
• 36 min
  7. Intrinsically unstructured proteins: regulation and disease

  • Dr. M. Madan Babu
• 43 min
  8. The roles of intrinsic disorder in protein interaction networks

  • Prof. Vladimir N. Uversky
• Gene Regulatory Networks and their Role in Protein Homeostasis
• 38 min
  9. Network motifs: basic building blocks of biological networks

  • Prof. Uri Alon
• 41 min
  10. Structure, evolution and dynamics of gene regulatory networks

  • Dr. M. Madan Babu
• 33 min
  11. Computational methods in analysis of gene regulation and protein interactions

  • Prof. Ron Shamir
• 51 min
  12. Protein-protein interaction networks

  • Prof. Peter Csermely
• Protein Homeostasis in the Endoplasmic Reticulum
• 56 min
  13. Role of calnexin and calreticulin in protein homeostasis within the endoplasmic reticulum

  • Prof. David B. Williams
• 30 min
  14. The unfolded protein response

  • Prof. Kazutoshi Mori
• 38 min
  15. Role of ER stress in cystic fibrosis airway inflammation

  • Dr. Carla Maria Pedrosa Ribeiro
• 57 min
  16. The recognition of misfolded glycoproteins in the endoplasmic reticulum

  • Dr. David Y. Thomas
• 49 min
  17. Chaperone systems of the endoplasmic reticulum

  • Prof. Linda M. Hendershot
• 74 min
  18. The ERAD network

  • Prof. Daniel Hebert
• Protein Homeostasis in the Mitochondria
• 22 min
  19. Protein homeostasis in mitochondria: AAA+ chaperones & proteases

  • Dr. David A. Dougan
• 46 min
  20. Protein homeostasis in mitochondria: chaperones and proteases of the mitochondrial matrix

  • Prof. Wolfgang Voos
• 26 min
  21. Mitochondrial chaperonin Hsp60: locations, functions and pathology

  • Prof. Francesco Cappello
  • Prof. Alberto J. L. Macario
• Protein Homeostasis in the Nucleus
• 23 min
  22. Nuclear protein quality control degradation

  • Dr. Richard G. Gardner
• Protein Homeostasis in Aging Disease
• 27 min
  23. Protein homeostasis during ageing: C. elegans as a model organism

  • Prof. Nektarios Tavernarakis
• Protein Homeostasis in Neurodegeneration
• 41 min
  24. Endoplasmic reticulum stress in neurodegenerative diseases

  • Prof. Claudio Soto
• 48 min
  25. Roles for Hsp40 molecular chaperones in protein misfolding disease

  • Prof. Douglas M. Cyr
• 38 min
  26. Protein folding in vivo

  • Prof. James Bardwell
• 39 min
  27. Protein degradation and defense against neurodegenerative disease 1

  • Prof. Alfred Goldberg
• 42 min
  28. Protein degradation and defense against neurodegenerative disease 2

  • Prof. Alfred Goldberg

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Concept of a molecular chaperone
3. Saccharomyces cerevisiae
4. TAP tag pull-downs (C-TAP)
5. Mapping chaperon interaction network in yeast
6. General principles: protein chaperon interaction
7. Top MIPS complexes interacting with chaperons
8. Top Pfam domains interacting with chaperons
9. Chaperon-chaperon interactions
10. Interactor overlap between two chaperones
11. Single vs. multiple pathways model (1)
12. Single vs. multiple pathways model (2)
13. New cellular pathways modulated by chaperones
14. Hsp90 and the conserved R2TP complex
15. Hsp90: overview
16. Integrated proteomics approaches
17. 2H Two-hybrid screen using an ordered array
18. TA tag pull-downs (forward and reverse)
19. SGA Synthetic lethal screen
20. GS Geldanamycin screen
21. Hsp90 putatively interacts with 627 proteins
22. Venn diagram
23. Hsp90 putatively interaction (1)
24. Hsp90 putatively interaction (2)
25. Origin of synthetically lethal proteins with HSP90
26. Proteomic data verification
27. Biochemistry of individual proteins/systems
28. Interesting ORF characteristics
29. Tah1 and Pih1
30. NMR structure of Tah1-MEEVD
31. Hsp90-Tah1-Pih1 form a ternary complex
32. Hsp90-Tah1-Pih1 complex function
33. Hsp90 maintains the stability of Pih1
34. Hsp90-Tah1 stabilize Pih1 in vivo (1)
35. Hsp90-Tah1 stabilize Pih1 in vivo (2)
36. Tah1 acts as a cofactor for HSP90
37. Tah1 & Pih1 target HSP90 to specific proteins
38. What is the function of the R2TP complex?
39. R2P role in the cell
40. Nop58 genetically interacts with R2TP
41. The simplified view
42. Box C/D snoRNP assembly
43. rRNA and snoRNA accumulation (1)
44. rRNA and snoRNA accumulation (2)
45. Proposed model
46. Summary
47. Funding
48. Thank you

Topics Covered

• Large scale proteomic screening in yeast
• The TAP-tag pulldown approach
• Understanding the principles governing protein homeostasis
• Mapping the network of the Hsp90 chaperone using physical and genetic interaction screens
• Unraveling the role of Hsp90 in pre-rRNA processing

Links

Series:

• Protein Homeostasis

Categories:

• Biochemistry
• Cell Biology
• Microbiology

Talk Citation

Publication History

• Published on February 2, 2012

Financial Disclosures

• Prof. Walid A. Houry has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.