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1. Introduction
2. Ebola virus
3. Ebola virus GP (glycoprotein)
4. GP structure
5. GP crystallization
6. Final crystallization construct
7. Physiological relevance
8. Final crystals
9. GP crystal structure
10. GP structure (360 view)
11. GP trimer interface
12. How is GP assembled?
13. GP1 and GP2 subdomains
14. GP1 base domain (1)
15. GP1 base domain (2)
16. GP1 head domain
17. GP1 glycan cap
18. GP2 N terminus
19. GP2 internal fusion loop
20. GP2 heptad repeat 1
21. Three HR1D subunits form the trimer interface
22. Three internal fusion loops wrap around the trimer
23. GP function in attachment, fusion and entry
24. GP residues important for attachment
25. Putative receptor binding sites
26. Glycan caps
27. Biantennary glycans
28. Mucin-like domain make GP 5-12 times bigger
29. When and how does the RBS come into play?
30. How is the RBS exposed?
31. Probable cathepsin cleavage site
32. After cleavage with cathepsin
33. Hypothesis for entry
34. Model for entry
35. What is left exposed for immune surveillance?
36. Survivor Fab KZ52 neutralizes in vitro
37. Three KZ52 Fabs bound to GP trimer
38. KZ52 is primarily an anti-GP2 antibody
39. Effect of KZ52
40. KZ52 bridges N term of GP2, IFL of GP2, and GP1
41. GP amino acids interacting with KZ52
42. Sudan ebolavirus (1)
43. Sudan ebolavirus (2)
44. Crystals of Sudan ebolavirus GP
45. Sudan ebolavirus GP bound to the 16F6 mAb
46. KZ52 and 16F6 binding sites overlap
47. How might Abs against the shared site neutralize?
48. What are those conformational changes?
49. Prefusion and postfusion GPs
50. Possible conformational changes at fusion (1)
51. Possible conformational changes at fusion (2)
52. Thanks

Topics Covered

• Structure of Zaire ebolavirus GP Receptor binding
• Cathepsin cleavage
• Immune evasion Structure of Sudan ebolavirus GP
• GP epitopes of two neutralizing antibodies
• Proposed conformational changes in fusion

Links

Categories:

• Biochemistry
• Immunology
• Microbiology

Therapeutic Areas:

• Immunology & Inflammation
• Infectious Diseases

Talk Citation

Ollmann Saphire, E. (2011, March 30). A shared structural solution for neutralizing ebolaviruses [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved May 1, 2024, from https://hstalks.com/bs/1980/.
Export Citation (RIS)

Publication History

• Published on March 30, 2011

Financial Disclosures

• Dr. Erica Ollmann Saphire has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.