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1. Introduction
2. Transcription factors in developmental hierarchies
3. Creation of three germ layers during gastrulation
4. Cell shape changes during gastrulation
5. Drosophila embryo gastrulation process
6. Genetic cascade regulating mesoderm creation
7. Twist is required for mesoderm development
8. Maternal factors in the epithelial cells
9. Zygotic factors in the epithelial cells
10. Early twist target genes and their functions
11. The snail target gene
12. The fog target gene
13. The string (cdc25) target gene
14. The FGF-R and dof adapter target genes
15. Snail and Fog are needed for invagination
16. Twist targets involved in ventral furrow formation
17. Twist targets
18. Gastrulation defects in embryos
19. Genes uncovered by the deficiencies
20. Mitotic domains
21. String/cdc25 expression is the limiting factor
22. The first domain to express String
23. Prospective mesoderm is the tenth to divide
24. Premature mitoses in mutant embryos
25. Is premature mitosis a cause or a result?
26. Results of suppressing premature mitoses
27. Tribbles mutants are viable
28. Df(3R)Tl-P target gene
29. Df(3R)Tl-P function
30. T48 is a twist target
31. T48 structure
32. Membrane localisation of T48 in epithelium
33. T48 has a potential PDZ binding motif
34. PDZ domain
35. Potential interactors for t48
36. RhoGEF necessary for myosin apical localisation
37. T48 binds to RhoGEF2
38. Testing for binding in S2 Schneider cells
39. T48 recruits RhoGEF2 to the plasma membrane
40. Do T48 and RhoGEF2 interact in the embryo?
41. RhoGEF and Armadillo in a bit older embryo
42. RhoGEF and Armadillo a few minutes later
43. Armadillo and rhoGEF in t48 mutants
44. T48 mutants have a mild phenotype and are viable
45. Gastrulation fails if both t48 and Fog are lost
46. Armadillo and rhoGEF in cta t48 double mutants
47. How does it all fit together?
48. Microtubule plus end associated rhoGEF
49. Effect of t48 and Fog on gastrulation process
50. Snail down regulates adherence junctions
51. RhoGEF and armadillo in snail mutants
52. Overview diagram I
53. Tribolium castaneum gastrulation
54. Acknowledgements

Topics Covered

• Events during gastrulation and mesoderm cell invagination
• Cell shape changes
• Genes acting upstream of mesodermal invagination
• Search for genes controlling mesoderm cell shape changes
• The role of cell division
• The importance of apical concentration of cytoskeletal regulators, and proteins mediating their apical concentration

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Reproduction & Development

Talk Citation

Publication History

• Published on September 29, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Dr. Maria Leptin has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.