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- Introduction
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1. Prions and amyloids: introduction
- Prof. Reed Wickner
- Mammalian Prions
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2. Transgenic mouse models of prion diseases
- Prof. Glenn Telling
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3. Mechanism of prion generation in vitro
- Dr. Surachai Supattapone
- Non-Prion Amyloids
- Yeast Prions
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6. Chaperones and prions
- Prof. Yury Chernoff
- Beneficial Amyloids
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7. The dark side of amyloid: PMEL, a natural amyloid in melanosome biogenesis
- Prof. Michael Marks
- Archived Lectures *These may not cover the latest advances in the field
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8. Predicting TSE transmission
- Prof. Jean Manson
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10. Yeast and fungal prions: a help or a hindrance?
- Prof. Reed Wickner
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11. [PIN+]: prions beget prions
- Prof. Susan Liebman
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12. Yeast prions and protein chaperones
- Dr. Daniel Masison
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13. Mechanisms of yeast prion propagation
- Prof. Mick Tuite
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14. Propagation and variability of the yeast [PSI+] prion
- Prof. Michael Ter-Avanesyan
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15. The genetics and biology of the [Het-s] prion of Podospora
- Prof. Sven Saupe
Printable Handouts
Navigable Slide Index
- Introduction
- Prions
- Prions are infectious amyloids
- Amyloid fibres growth process
- Fragmentation of prion polymers
- Important questions concerning prions
- In vivo approach - prion propagation mechanism
- Yeast prion propagation and rapid cell division
- The PSI+ prion and its associated phenotype
- The prion protein Sup35p: eRF3 (1)
- Yeast prions are efficiently propagated
- Prion seeds and the 'propagon'
- The molecular chaperone Hsp104 discovery
- The molecular chaperone Hsp104 discovery
- Propagation of PSI+ prion and the role of Hsp104
- Hsp104 activity Inhibition leads to propagons loss
- Guanidinium ion is an Hsp104-specific inhibitor
- GdnHCl eliminates [PSI+] prion from growing cells
- GdnHCl-induced [PSI+] curing and cell division (1)
- GdnHCl-induced [PSI+] curing and cell division (2)
- Calculating propagon numbers
- Proportion of propagons passed to daughter (1)
- Proportion of propagons passed to daughter (2)
- The advanced stochastic model
- Mechanism of new propagon generation
- The generation of new propagons
- Hsp104 elevated levels block [PSI+] propagation
- Isolation and genetic behaviour of PNM mutants
- PNM1 alleles of HSP104 show a defect in function
- The prion protein Sup35p: eRF3 (2)
- The prion protein Sup35p: eRF3 (3)
- PNM2-1 encodes a Sup35p defective form
- The effect of PNM2-1 mutation on propagation
- Sup35p primary sequence and [PSI+] propagation
- Other amino acid substitutions at Gly58
- PNM2-1 mutation reduces PFD flexibility
- An overview of [PSI+] prion propagation
- Summary
- Acknowledgements
- References
Topics Covered
- Prions as infectious amyloids
- Yeast [PSI+] prion and Sup35p
- Molecular chaperone Hsp104
- Guanidine hydrochloride as a prion propagation inhibitor
- Propagons
- Estimating propagon numbers using a stochastic model
- Generation of new propagons
- Propagation-defective mutants (PNM)
- Role of primary sequence information
- Yeast prion propagation: the unanswered questions
Talk Citation
Tuite, M. (2008, September 4). Mechanisms of yeast prion propagation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/HVDH6156.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Mick Tuite has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.