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1. Introduction
2. Ca is a key signaling molecule in the cell
3. The revival of protein-based Ca probes
4. Protein based fluorescent calcium probes: aequrin
5. Protein based fluorescent calcium probes: GFP
6. Ca accumulation in mitochondrial matrix (1)
7. Ca accumulation in mitochondrial matrix ( 2)
8. Ca diffusion through OMM: VDAC
9. The VDAC repertoire is rate-limiting
10. Mitochondrial Ca uptake in VDAC overexpression
11. VDAC shortens the mitochondrial Ca uptake delay
12. Ca transport across the inner membrane
13. The basic principles of inner membrane transport
14. Effect of membrane potential on Ca equilibrium
15. What prevents mitochondrial Ca overload?
16. Why is Ca uptake fast in stimulated cells?
17. The mitochondrial calcium uniporter (mCU)
18. The return of Ca to basal values
19. Ca uptake and regulation of aerobic metabolism
20. Luciferase: a gene-encoded ATP probe
21. Ca can lead to an increased ATP production
22. Ca increased and mitochondria ATP production
23. Ca uptake and activation of apoptotic process
24. Ca synergizes with apoptotic stimuli
25. Ca signals reduction by Bcl-2
26. Ca uptake and the apoptotic process
27. HBx induces Ca mitochondrial rise
28. Roles of mitochondrial Ca uptake: summery
29. Modulation of [Ca] microdomains
30. Pancreatic acinar cells: mitochondrial firewall
31. Summary

Topics Covered

• Targeted Calcium indicators
• The principles of mitochondrial Calcium accumulation
• The role of the outer membrane channel VDAC
• The mitochondrial Calcium uniporter of the inner membrane (MCU)
• The ER-mitochondria contacts
• Calcium re-extrusion
• ATP measurements
• The role of Calcium in mitochondrial functions (aerobic metabolism and apoptosis induction)

Links

Series:

• Calcium Signaling I
• The Endoplasmic Reticulum

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Rizzuto, R. (2007, October 1). Regulation and role of mitochondrial Ca2+ homeostasis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/LYSN8890.
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Publication History

• Published on October 1, 2007

Financial Disclosures

• Prof. Rosario Rizzuto has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.