• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Acknowledgements
3. Haloarcula marismortui is not a human pathogen
4. Antibiotic - first definition
5. Antibiotic - a broader definition
6. Penicillin
7. Paradox
8. Antibiotic specificity problems
9. Differences between ribosomes sensitivity
10. Good news
11. Bad news
12. A complete 70S ribosome
13. Large subuint
14. A fifth active site at the large ribosimal subunit
15. Structures of anti-ribosomal antibiotics
16. Binding of puromycin in the large subunit
17. Binding of anisomycin in the large subunit
18. Binding of sparsomycin in the large subunit
19. Binding of blasticidin in the large subunit
20. Binding of tylosin in the large subunit
21. Large/small subunit antibiotics
22. Anisomycin binding to the A cleft
23. Anisomycin cause conformation change
24. Position of U2539
25. Macrolide antibiotics
26. Ribosome-antibiotic complexes
27. Resistance to macrolide antibiotic
28. Position 2099 identity is significant
29. Azithromycin
30. Differences in peptide length product
31. Textbook explanation

Topics Covered

• Introduction to a paradox: how can a universally conserved cellular component be a target for pharmacologically useful antibiotics?
• Introduction to ribosome structure
• Antibiotic binding sites cluster in the ribosome
• The source of the species specificity of antibiotics that interact with the A-site cleft
• How macrolides interact with the ribosome
• The structural consequences of mutations that make ribosomes resistant to macrolides
• The toxicity of macrolides is not well understood
• 13-deoxytedanolide, a novel macrolide with novel species specificity that binds to a novel site in the ribosome

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Microbiology
• Pharmaceutical Sciences

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on February 25, 2021

Financial Disclosures

• Prof. Peter Moore has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.