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1. Introduction
2. Elongation of polypeptide chain by one aa
3. Two GTPases in the elongation cycle
4. EF-G, ternary complex: molecular mimicry
5. The factor binding site on the ribosome
6. Mapping binding sites of EF-Tu, EF-G, and tRNA
7. Elongation cycle of protein synthesis
8. Decoding in two steps - introduction
9. Structural aspects of initial tRNA selection (1)
10. Structural aspects of initial tRNA selection (2)
11. Structural aspects of initial tRNA selection (3)
12. EF-Tu departure upon GTP hydrolysis
13. The ternary complex after GTP hydrolysis
14. tRNA selection and accommodation
15. Conformation of aa-tRNA in the A/T state
16. Proofreading and accommodation (1)
17. Proofreading and accommodation (2)
18. Proofreading and accommodation (3)
19. aa-tRNA accommodation and peptidyl-transfer
20. Translocation
21. mRNA-tRNA translocation in two steps
22. Translocation step I: ratchet motion
23. EF-G conformation upon ribosom binding
24. “Induced fit” in EF-G and the ribosome
25. Ratchet motion and translocation
26. L2-S6 crosslink inhibits translocation
27. FRET studies (1)
28. FRET studies (2)
29. Translocation of tRNA entails “hybrid states”
30. Effects of the ratchet motion
31. Basic concept of translocation
32. Questions concerning the basic model
33. Mechanism for closing and opening the lock
34. Restacking of bases A1492/A1493
35. Additional rotation of the small subunit
36. Observed small subunit head rotation
37. mRNA-tRNA translocation model: summary (1)
38. mRNA-tRNA translocation model: summary (2)
39. Conclusions

Topics Covered

• Schematic of elongation cycle
• Role of elongation factors EF-G and EF-Tu
• Molecular mimicry of elongation factors
• Binding positions of factors and tRNA during elongation cycle
• Process of tRNA selection
• Proofreading
• Role of bases A1492 and A1493
• GTP hydrolysis on EF-Tu
• tRNA as a molecular spring
• Translocation
• Intersubunit ratchet motion
• Induced fit
• FRET studies of ratchet motion
• Hybrid states of tRNA
• Role of GTP hydrolysis in translocation

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry

Talk Citation

Frank, J. (2008, May 15). Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/YJIX1530.
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Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Joachim Frank has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.