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1. Introduction
2. Foundmental processes during protein synthesis
3. Peptidyl transfer catalysis by RNA-rich active site
4. Ribosome structure solution provided new insights
5. Peptide bond formation and peptide release
6. Active site of the ribosome
7. Universally conserved nucleotides of the active site
8. Affinity tag purification of mutant ribosomes
9. Pre-steady state assay for peptide bond formation
10. Active site mutations and rates of peptidyl transfer
11. A-site substrates: tRNA vs. puromycin
12. Active site variants recovery by C75 addition to Pm
13. "Uninduced" state of ribosome and P-site substrate
14. A-site substrates-induced conformational changes
15. Peptide release activity of variant ribosomes
16. How do release factors function?
17. RF catalysis in the large subunit
18. Other RF conserved amino acids in bacteria
19. Consequences of RF1 site-directed mutations
20. How can we make substitution in water?
21. Nucleophile partitioning provides a handle
22. Mutants that appear to have lost water specificity
23. Permissivity and size of side chain at position 235
24. RF components functioning in activating hydrolysis
25. Summary
26. Regulation of translation post-initiation

Topics Covered

• Fundamental processes during protein synthesis
• Peptidyl transfer catalysis by RNA-rich active site
• Ribosome structure solution provided new insights
• Peptide bond formation and peptide release
• Active site of the ribosome
• Universally conserved nucleotides of the active site
• Affinity tag purification of mutant ribosomes
• Active site mutations and rates of peptidyl transfer
• A-site substrates: tRNA vs. puromycin
• "Uninduced" state of ribosome and P-site substrate
• A-site substrates-induced conformational changes
• Peptide release activity of variant ribosomes
• RF catalysis in the large subunit
• RF conserved amino acids in bacteria
• Consequences of RF1 site-directed mutations
• Nucleophile partitioning
• Mutants that lost water specificity
• RF components functioning in activating hydrolysis
• Regulation of translation post-initiation

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Rachel Green has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.