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Navigable Slide Index

1. Introduction
2. The central dogma
3. tRNA is the central player in translation
4. The probability of errors in protein synthesis
5. Selectivity and transition state
6. The genetic code
7. Decoding - selection of the correct tRNA
8. The bacterial ribosome
9. Antibiotics change the fidelity of the ribosome
10. Fidelity mutations far from the anticodon
11. Selection of aa-tRNA by the ribosome (1)
12. Problems with the tRNA selection scheme
13. Selection of aa-tRNA by the ribosome (2)
14. The atomic structure of the 30S subunit
15. First clue of how decoding might work
16. Testing the decoding model
17. Induced fit on tRNA binding
18. Minor groove recognition
19. Near-cognate Leu(II) ASL in the 30S
20. Structures with cognate and near-cognate ASL
21. Binding of Leu(II) tRNA
22. Tautomers and base pairs
23. An unusual tautomer at 2nd position?
24. 30S closing movement
25. Ribosome mutations that affect accuracy
26. Energetics of transition to closed form
27. Domain closure leads to tRNA selection
28. tRNA orientations
29. TC loop / tRNA summary
30. An integrated view of decoding
31. Base pairing and fidelity
32. Synchrotrons
33. Acknowledgements

Topics Covered

• tRNA: the central player in translation
• Selectivity and transition state
• Decoding: selection of the correct tRNA
• The bacterial ribosome
• Antibiotics change the fidelity of the ribosome
• Selection of aa-tRNA by the ribosome
• The atomic structure of the 30S subunit
• Testing the decoding model
• Minor groove recognition of base pairing geometry
• Tautomers and base pairs
• Streptomycin
• Base pairing and fidelity

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Ramakrishnan, V. (2008, May 15). Structural insights into decoding of mRNA by the ribosome [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 15, 2025, from https://doi.org/10.69645/NWAT9316.
Export Citation (RIS)

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Venki Ramakrishnan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.