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Navigable Slide Index

1. Introduction
2. DNA plasmids
3. The yeast plasmid
4. Plasmid amplification
5. Fluorescence-tagged 2 micron plasmid
6. Plasmid dynamics during the cell cycle
7. Chromosome and plasmid segregation
8. Cell cycle and cohesin-STB association
9. Cohesin cleavage and plasmid partitioning
10. CSE4-STB association
11. Cohesin-STB association in the CSE4 mutant
12. Protein association at STB at G1-S
13. Pathway for equal plasmid segregation
14. Cohesion in the 2 micron plasmid
15. Plasmid pairing is cohesin-mediated
16. Cohesin-mediated sister-to-sister counting
17. Random grouping of replicated plasmids
18. STB-STB segregation
19. CEN-CEN segregation
20. CEN-STB segregation
21. Summary

Topics Covered

• The yeast plasmid as a highly optimized design for a multi-copy selfish DNA element
• The need for an active partitioning system for the segregation of a high copy plasmid
• Coupling between plasmid segregation and chromosome segregation
• Poaching of host factors required for chromosome segregation by the plasmid partitioning system
• Cohesin mediated equal segregation of duplicated plasmid clusters

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Jayaram, M. (2008, January 28). The yeast plasmid: a highly persistent selfish DNA element [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 17, 2025, from https://doi.org/10.69645/LITN8042.
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Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. Makkuni Jayaram has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.