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- Introduction and General Properties of Plasmids
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31 min
1. Introduction to plasmid biology- Prof. Emeritus Don Clewell
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39 min
2. General properties and applications of plasmids- Prof. Emeritus Don Clewell
- Replication
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47 min
3. Plasmids: copy number control by antisense RNAs- Prof. Gerhart Wagner
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30 min
4. Plasmid rolling-circle (RC) replication- Prof. Saleem Khan
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45 min
5. Replication of linear plasmids in bacteria- Prof. George Chaconas
- Maintenance and Partitioning
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32 min
6. Plasmid segregation and stability in bacteria- Prof. Barbara Funnell
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33 min
7. Plasmid stabilization by cell killing- Prof. Keith Weaver
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30 min
8. Dimer catastrophes and plasmid resolution- Dr. David Summers
- Conjugation and Mobilization
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41 min
9. The diversity of bacterial conjugation and mobilization systems- Prof. Fernando de la Cruz
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36 min
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27 min
11. DNA processing in conjugative transfer- Dr. Joel Schildbach
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43 min
12. Agrobacterium tumefaciens- Dr. Stephen Winans
- Genetic Traits Frequently Associated with Plasmids
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37 min
13. Insertion sequences and DNA transposition- Prof. William Reznikoff
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48 min
14. Plasmids, integrons and the spread of antibiotic resistance- Prof. Hatch Stokes
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30 min
15. Virulence plasmids- Prof. Julian Rood
- Eukaryotic Plasmids
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30 min
16. The yeast plasmid: a highly persistent selfish DNA element- Prof. Makkuni Jayaram
- Population Biology and Evolution
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35 min
17. Evolution of plasmids and their role in bacterial diversity and adaptability- Prof. Christopher Thomas
- Archived Lectures *These may not cover the latest advances in the field
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85 min
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34 min
19. Plasmid segregation and stability in bacteria- Prof. Barbara Funnell
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39 min
20. Replication of linear plasmids in bacteria- Prof. George Chaconas
Printable Handouts
Navigable Slide Index
- Introduction
- Plasmid partition and chromosome dynamics
- Plasmid stability systems
- Why do plasmids need partition systems?
- Plasmid localization in the cell cycle
- Plasmid partition cassettes
- Classes of partition systems
- Type 1: walker ATPases
- Plasmid partition sites (1)
- Plasmid partition sites (2)
- Repressor and corepressors
- The diversity of plasmids with type 1 par systems
- Type 2: actin ATPases
- Plasmids with multiple par systems
- Mechanistic questions
- The action of ParA and ParB proteins: ParB
- The P1 partition complex
- Visualization of partition complexes
- ParB structure and function
- The structure of a partition complex
- ParBs of Type 1 par systems
- Small ParBs are RHH2 dimers
- Omega RHH2 binds DNA
- The action of ParA and ParB proteins: ParA
- Plasmid localization requires ParA
- Measurements of localization
- Walker-type partition ATPases
- ParA binds and hydrolyzes ATP
- ParA is a transcriptional repressor
- ParA has site-specific DNA binding activity
- Mutations in ParA reveal ATP interactions
- The ATP-ADP switch
- How does ParA mediate plasmid localization?
- ParM and actin share properties
- ParM filaments drive plasmids apart
- ParM polymerization drives R1 partition
- Do walker-type ATPases polymerize?
- Partition ATPase dynamics: observations
- Model: par complex dynamics
- Remaining questions
Topics Covered
- Mechanisms of plasmid segregation and partition reactions
- Types of plasmid partition systems in bacteria
- ParA partition ATPases
- Structural biology of partition-site binding proteins
- Chromosome dynamics
Links
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Talk Citation
Funnell, B. (2008, January 28). Plasmid segregation and stability in bacteria [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 26, 2024, from https://doi.org/10.69645/ZFPH4100.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no commercial/financial matters to disclose.
