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1. Introduction
2. Linear DNA in diverse bacteria
3. The "end replication" problem
4. Solving the end replication problem
5. Streptomyces
6. Replication pathway for adenovirus and Bacillus
7. Streptomyces DNA replication
8. A minimal pSLA2 replicon
9. pSLA2 replication - cis and trans acting regions
10. Linear DNA replication intermediate
11. Secondary structure of SLP2 plasmid end
12. Patching process mediated by terminal protein
13. The terminal proteins of Streptomyces
14. Comparison of seven TP sequences
15. Deoxynucleotidylation of TP
16. Recruitment of TP
17. Streptomyces summary
18. Borrelia
19. The segmented genome of Borrelia burgdorferi
20. Borrelia linear plasmids replication pathway (1)
21. Origin mapping by nascent strand analysis
22. Origin location in the linear chromosome
23. Plasmid maintenance proteins in B. burgdorferi
24. Minimal replicons
25. Regions required for lp17 replication
26. The telomere resolvase ResT
27. Borrelia linear plasmids replication pathway (2)
28. Purification of recombinant B. burgdorferi ResT
29. Assay for telomere resolution by ResT
30. The substrate
31. Three classes of telomere in B. burgdorferi
32. Minimal substrate requirements
33. Position of the cleavage site in telomeres
34. How does DNA cleavage and ligation occur?
35. ResT action mechanism
36. Alignment of telomere resolvases
37. Features of telomere resolvases
38. How does hairpin formation occur?
39. Alignment of hairpin binding modules with ResT
40. A composite active site and telomere resolution
41. A composite active site
42. Genome plasticity in linear Borrelia plasmids
43. Sequence scrambling in linear plasmids
44. Telomere exchanges
45. DNA rearrangements in B. burgdorferi
46. ResT reversal using a plasmid substrate
47. ResT-mediated telomere fusions
48. Sequence scrambling near telomeres
49. Reversal of telomere resolution
50. Conclusions - genome plasticity and ResT

Topics Covered

• The end replication problem and solutions
• Replication of linear DNA in Streptomyces species using terminal proteins
• Replication of linear DNA in Borrelia species using telomere resolution
• Genome plasticity in Borrelia resulting from reversal of telomere resolution

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Prof. George Chaconas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.