• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Outline
3. The plasmid lifestyle
4. Plasmids don't want to get lost
5. Plasmids run a high risk of getting lost
6. Negative feedback control of plasmid replication
7. Plasmid R1
8. General properties
9. Copy number control of plasmid R1
10. Indirect inhibition and translational coupling
11. Interaction between folded RNAs
12. Copy number control of ColE1-type plasmids
13. Regulation by induced RNA folding change
14. Copy number control of plasmid pIP501
15. RNAIII induces transcription termination
16. Copy number control of IncI-alpha/IncB plasmids
17. Inhibition of activator pseudoknot
18. Different mechanisms - same principle
19. Kinetic control of copy number
20. Binding pathways - a hierarchy of steps
21. The CopA/CopT four helix junction structure
22. Special tricks for fast RNA-RNA recognition
23. Helper proteins
24. Acknowledgements

Topics Covered

• Negative feedback control of plasmid replication
• General properties of plasmids
• Plasmid R1
• Copy number control of plasmid R1
• Inhibition and translational coupling
• Interaction between folded RNAs
• Copy number control of ColE1-type plasmids
• Regulation by induced RNA folding change
• Copy number control of plasmid pIP501
• RNAIII induces transcription termination
• Copy number control of IncIa/IncB plasmids
• Inhibition of activator pseudoknot
• Kinetic control of copy number
• Binding pathways: a hierarchy of steps
• The CopA/CopT four helix junction structure
• Fast RNA-RNA recognition
• Helper proteins

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008
• Reviewed on April 30, 2023

Financial Disclosures

• Prof. Gerhart Wagner has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.